LOCUS       sequence-158026-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes TET1 under LITE2.0 system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Lo CL, Choudhury SR, Irudayaraj J, Zhou FC
  TITLE     Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by 
            Optogenetics.
  JOURNAL   Sci Rep. 2017 Feb 9;7:42047. doi: 10.1038/srep42047.
  PUBMED    28181538
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-02-9"; volume: "7:42047. doi"; pages: " 
            10.1038/srep42047"
COMMENT     Sequence Label: TET1CD-CRY2-EGFP
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..72)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(94..243)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(268..288)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcctcgccc ttgctcactg ggccaggatt ctcctcgacg tcaccgcatg ttagcagact
       61 tcctctgccc tctccactgc ctctagagtt aatcagcatg tccaggtcga aatcatcaag
      121 ggcgtcactg ccgagcatgt cgaggtcaaa gtcatcaagg gcatccgaac caagcatgtc
      181 aaggtcaaaa tcatcgaggg cgtcacttcc cagcatatcc agatcaaaat cgtccaatgc
      241 gtcagcccgt ccggaaccgc tggcctccac ctttctcttc ttcttggggc tagccggcgc
      301 tgctccgatc ataatctgcg cttcgcgtgt tcttgagatg gctttcgcca gcaattctcg
      361 tgcggtgtca atatcgacga tgggtttcgc gtaattcgtc cccaattcga cccctgaggc
      421 tttaaggacg gtcaggggag catcccacgg gtggtgaatc cactcggtgg gcagacgggc
      481 cagctctggg agccactgcc tgatgtattc tccctccggg tcgtactttg ctccttgcag
      541 tgcagggtta tccaatcggt caagttcatg cccgtcaggg atggaacccg aaatgtactg
      601 ccacccaaga atatcgcact ccaagtccgc atccagaagc gtgtcccaaa agtatttcat
      661 cccccatttc cagggcaaca agaggaactt aacggcaaag ctactaacga tgactcgaat
      721 acggttgtgc atccaacccg ttgcccacag ctctctcata cccgcatcca ccaacgggta
      781 tcccgtgcgc ccctgccgcc aggccttgaa tttgtccacg tccgcgtccc acggaaagaa
      841 gcggaggtgg ctgaggaggg attgttcatg tgtaaacgga aagttgaagc agatgtatcg
      901 tgaatactcc ctaaggccga ttcctctcaa aaacaaatcg gcagactcct ctccttcaga
      961 gtttttgtcg cgagcccaaa tgatttgctt
//
