LOCUS       sequence-158028-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes DMNT3 under LITE2.0 system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Lo CL, Choudhury SR, Irudayaraj J, Zhou FC
  TITLE     Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by 
            Optogenetics.
  JOURNAL   Sci Rep. 2017 Feb 9;7:42047. doi: 10.1038/srep42047.
  PUBMED    28181538
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-02-9"; volume: "7:42047. doi"; pages: " 
            10.1038/srep42047"
COMMENT     Sequence Label: DNMT3ACD-CRY2-EGFP
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..73)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(95..244)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(269..289)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat gttagcagac
       61 ttcctctgcc ctctccactg cctctagagt taatcagcat gtccaggtcg aaatcatcaa
      121 gggcgtcact gccgagcatg tcgaggtcaa agtcatcaag ggcatccgaa ccaagcatgt
      181 caaggtcaaa atcatcgagg gcgtcacttc ccagcatatc cagatcaaaa tcgtccaatg
      241 cgtcagcccg tccggaaccg ctggcctcca cctttctctt cttcttgggg ctagccggcg
      301 ctgctccgat cataatctgc gcttcgcgtg ttcttgagat ggctttcgcc agcaattctc
      361 gtgcggtgtc aatatcgacg atgggtttcg cgtaattcgt ccccaattcg acccctgagg
      421 ctttaaggac ggtcagggga gcatcccacg ggtggtgaat ccactcggtg ggcagacggg
      481 ccagctctgg gagccactgc ctgatgtatt ctccctccgg gtcgtacttt gctccttgca
      541 gtgcagggtt atccaatcgg tcaagttcat gcccgtcagg gatggaaccc gaaatgtact
      601 gccacccaag aatatcgcac tccaagtccg catccagaag cgtgtcccaa aagtatttca
      661 tcccccattt ccagggcaac aagaggaact taacggcaaa gctactaacg atgactcgaa
      721 tacggttgtg catccaaccc gttgcccaca gctctctcat acccgcatcc accaacgggt
      781 atcccgtgcg cccctgccgc caggccttga atttgtccac gtccgcgtcc cacggaaaga
      841 agcggaggtg gctga
//
