LOCUS       sequence-158029-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tet inducible bidirectional plasmid expressing GFP in one direction 
            and in the other a human DMPK genomic segment containing exons 11-15
            with 960 interrupted CTG repeats in exon 15 located.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Lee JE, Bennett CF, Cooper TA
  TITLE     RNase H-mediated degradation of toxic RNA in myotonic dystrophy type
            1.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4221-6. doi: 
            10.1073/pnas.1117019109. Epub 2012 Feb 27.
  PUBMED    22371589
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1117019109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-03-13- 13"; volume: "109"; issue: "11"; pages: "4221-6"
COMMENT     Sequence Label: pBItetDT960GFP
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(9..722,723..725,726..728))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(12..722,723..725,726..728))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     403..422
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     662..683
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      725..734
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
       61 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      121 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      181 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      241 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      301 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      361 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      421 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      481 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
      541 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      601 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
      661 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
      721 ctcaccatgg tggcgaccgg cgcgtcagct gactagagga tccccgggta ccgagc
//
