LOCUS       sequence-158031-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tet inducible bidirectional plasmid expressing GFP in one direction 
            and in the other a human DMPK genomic segment containing exons 11-15
            with 960 interrupted CTG repeats in exon 15 located.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Lee JE, Bennett CF, Cooper TA
  TITLE     RNase H-mediated degradation of toxic RNA in myotonic dystrophy type
            1.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4221-6. doi: 
            10.1073/pnas.1117019109. Epub 2012 Feb 27.
  PUBMED    22371589
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1117019109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-03-13- 13"; volume: "109"; issue: "11"; pages: "4221-6"
COMMENT     Sequence Label: pBItetDT960GFP
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aataggcgta tcacgaggcc ctttcgtctt cactcgacgc ggccagcttg gtcgagctgg
       61 atacttcccg tccgccaggg ggacatgccg gcgatgctga aggtcgcgcg cattcccgat
      121 gaagaggccg gttactcgag cgcaggcgca gtggaaggag gatggccgcg cgcgctgcca
      181 gcccagcccc ctcttctcga cgctcggtgg cacagctggg ccacagctgg gcgggggcgg
      241 tgcctccggg tggcccgctc gccctcctat tggccggacg ccaaagcccc gccccgtggc
      301 ttttcctccc ccaaccctga ttcggccgct tcgcatcccg ctagctcctc ccagaccttc
      361 ggccgcctcc acacgcctcc ggattggccc gctgcggagc ctccggccca caacgcaaac
      421 cgcggacact gtggagtcca gagctttggg cagatggagg gccttttatt cgcgagggtc
      481 gggggtgggg gtcctaggtg gggacagaca atagataccg aggaatgtcg gggtctcagt
      541 gcatccaaaa cgtggattgg ggttgttggg ggtcctgtag cctgtcagcg agtcggagga
      601 cgaggtcaat aaatatccaa accgccgaag cgggcggggc cggctggggc tccgagagca
      661 gcgcaagtga ggaggggggc gcgggatccc cgaaaaagcg ggtttggcaa aagcaaattt
      721 cccgagtaag caggcagaga tcgcgccaga cgctccc
//
