LOCUS       sequence-158034-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episome vector for Phaeodactylum tricornutum (pBR322-based).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  TITLE     pPtPBR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPtPBR1
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..684)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 agtccagact cctgtgtaaa actacataag aacacctttg gtggagggaa catcgttggt
       61 accattgggc gaggtggctt ctcttatggc aaccgcaaga gccttgaacg cactctcact
      121 acggtgatga tcattcttgc ctcgcagaca atcaacgtgg agggtaattc tgctagcctc
      181 tgcaaagctt tcaagaaaat gcgggatcat ctcgcaagag agatctccta ctttctccct
      241 ttgcaaacca agttcgacaa ctgcgtacgg cctgttcgaa agatctacca ccgctctgga
      301 aagtgcctca tccaaaggcg caaatcctga tccaaacctt tttactccac gcacggcccc
      361 tagggcctct ttaaaagctt gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc
      421 gtctatgtgt aagtcaccaa tgcactcaac gattagcgac cagccggaat gcttggccag
      481 agcatgtatc atatggtcca gaaaccctat acctgtgtgg acgttaatca cttgcgattg
      541 tgtggcctgt tctgctactg cttctgcctc tttttctggg aagatcgagt gctctatcgc
      601 taggggacca ccctttaaag agatcgcaat ctgaatcttg gtttcatttg taatacgctt
      661 tactagggct ttctgctctg tcatctttgc cttcgtttat cttgcctgct cattt
//
