LOCUS       sequence-158039-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     86..105
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(195..218)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(225..257)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(297..320)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(327..347)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(354..371)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(390..566)
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
ORIGIN
        1 ttccaacaca ctattgcaat gaaaataaat ttcctttatt agccagaagt cagatgctca
       61 aggggcttca tgatgtcccc ataatttttg gcagagggaa aaagatcgat ctcagtggta
      121 tttgtgagcc agggcattgg ccacaccagc caccaccttc tgataggcag cctgcacctg
      181 aggttaatca cttagtgatg gtgatggtga tggtggtgct cgagatcctc ggggtcttcc
      241 ggggcgagtt ctggctggct tgcacgtgta tacagctgtg cggccgcaag cttttatttg
      301 tcgtcgtcgt ccttgtagtc gcggccgccc tggaaataca gattttcacc gccatgatga
      361 tgatgatgat ggctaccacc agaaccacct tttggggcct gggcatcgtt cagttttttg
      421 gcttcggcca gcagattggc gctctgagat ggatcatcaa acaggctcac gatgaaggca
      481 aacttctgct caacattcag gtttggcaga gaatggattt ccgcaccggc acgcgtcttt
      541 tctttattga atttgttatc caccatggat ccagaaccct tgtacagctc gtccatgccg
      601 ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg
      661 tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc
      721 ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc
      781 ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc
      841 tcccagccca tagtcttctt ctgcattaca gggccgtcgg aggggaagtt ggtgccgcgc
      901 agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc
      961 acgccgccgt cctcgaagtt catcacgcgc tcccactt
//
