LOCUS       sequence-158041-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of 
            human CtIP resistant to a siRNA. Confers resistance to puromycin. 
            Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-CtIP-siR-WT
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             175..201
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccggc aagcttgcgg ccgccgccac catgtacccc
      181 tacgatgtgc ctgactacgc cggatccatg aacatctcgg gaagcagctg tggaagccct
      241 aactctgcag atacatctag tgactttaag gacctttgga caaaactaaa agaatgtcat
      301 gatagagaag tacaaggttt acaagtaaaa gtaaccaagt tgaagcagga acgaatctta
      361 gatgcacaaa gactagaaga attcttcacc aaaaatcaac agctgaggga acagcagaaa
      421 gtccttcatg aaaccattaa agttttagaa gatcggttaa gagcaggctt atgtgatcgc
      481 tgtgcagtaa ctgaagaaca tatgcggaaa aaacagcaag agtttgaaaa tatccggcag
      541 cagaatctta aacttattac agaacttatg aatgaaagga atactctaca ggaagaaaat
      601 aaaaagcttt ctgaacaact ccagcagaaa attgagaatg atcaacagca tcaagcagct
      661 gagcttgaat gtgaggaaga cgttattcca gattcaccga taacagcctt ctcattttct
      721 ggcgttaac
//
