LOCUS       sequence-158043-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged RhoA Q63L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-RhoA Q63L
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..35
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    35..59
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        75..184
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             208..912
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(350..368)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggatcggacc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
       61 cggagttaat ccgggacctt taattcaacc caacacaata tattatagtt aaataagaat
      121 tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca ttttatttac
      181 aatcaaagga gatataccat gtctagagtg agcaagggcg aggaggataa catggccatc
      241 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      301 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      361 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      421 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      481 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      541 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      601 aacttcccct ccgacggccc tgtaatgcag aagaagacta tgggctggga ggcctcctcc
      661 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
      721 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      781 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
      841 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      901 gagctgtaca agggttctgg aagttccatg gtggataaca aattcaataa agaaa
//
