LOCUS       sequence-158044-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged RhoA Q63L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-RhoA Q63L
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..97)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(104..136)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(join(173..565,566..568,569..754))
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     CDS             complement(785..961)
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
ORIGIN
        1 ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga
       61 ggttaatcac ttagtgatgg tgatggtgat ggtggtgctc gagatcctcg gggtcttccg
      121 gggcgagttc tggctggctt gcacgtgtat acagctgtgc ggccgcaagc ttttacaaga
      181 caaggcaccc agattttttc ttcccacgtc tagcttgcag agcagctctc gtagccattt
      241 caaaaacctc tctcactcca tctttggtct ttgctgaaca ctccatgtac ccaaaagcgc
      301 caatcctgtt tgccatatct ctgccttctt caggtttcac cggctcctgc ttcatcttgg
      361 ctagctcccg ccttgtgtgc tcatcattcc gaagatcctt cttattccca accaggatga
      421 tgggcacgtt gggacagaaa tgcttgactt ctggggtcca cttttctggg atgttttcta
      481 aactatcagg gctgtcgatg gaaaaacaca tcagtataac atcggtatct gggtaggaga
      541 ggggcctcag gcgatcataa tcttccagcc cagctgtgtc ccacaaagcc aactctacct
      601 gctttccatc cacctcgata tctgccacat agttctcaaa cactgtgggc acatacacct
      661 ctgggaactg gtccttgctg aagactatga gcaagcatgt ctttccacag gctccatcac
      721 caacaatcac cagtttcttc cggatggcag ccatggtacc accagaacca gaaccaccgg
      781 atccttttgg ggcctgggca tcgttcagtt ttttggcttc ggccagcaga ttggcgctct
      841 gagatggatc atcaaacagg ctcacgatga aggcaaactt ctgctcaaca ttcaggtttg
      901 gcagagaatg gatttccgca ccggcacgcg tcttttcttt attgaatttg ttatccacca
      961 tggaacttcc agaacccttg tacagctcgt ccatgcc
//
