LOCUS       sequence-158056-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxy-inducible expression of 
            S664A+S679A+S745A human CtIP resistant to a siRNA. Confers 
            resistance to puromycin. Use T-REx cells for doxycycline-inducible 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-CtIP-siR-S664A-S679A-S745A
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..23
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     54..78
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             170..196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
       61 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      121 caccgggacc gatccagcct ccggcaagct tgcggccgcc gccaccatgt acccctacga
      181 tgtgcctgac tacgccggat ccatgaacat ctcgggaagc agctgtggaa gccctaactc
      241 tgcagataca tctagtgact ttaaggacct ttggacaaaa ctaaaagaat gtcatgatag
      301 agaagtacaa ggtttacaag taaaagtaac caagttgaag caggaacgaa tcttagatgc
      361 acaaagacta gaagaattct tcaccaaaaa tcaacagctg agggaacagc agaaagtcct
      421 tcatgaaacc attaaagttt tagaagatcg gttaagagca ggcttatgtg atcgctgtgc
      481 agtaactgaa gaacatatgc ggaaaaaaca gcaagagttt gaaaatatcc ggcagcagaa
      541 tcttaaactt attacagaac ttatgaatga aaggaatact ctacaggaag aaaataaaaa
      601 gctttctgaa caactccagc agaaaattga gaatgatcaa cagcatcaag cagctgagct
      661 tgaatgtgag gaagacgtta ttccagattc accgataaca gccttctcat tttctggcgt
      721 taaccggcta cgaagaaagg agaaccc
//
