LOCUS       sequence-158061-        2742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human mutant PC5A 
            (H516P) with C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX304 PCSK5 (H516P)-V5 blast
FEATURES             Location/Qualifiers
     source          1..2742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggctggg ggagccgctg ctgctgcccg ggacgtttgg acctgctgtg cgtgctggcg
       61 ctgctcgggg gctgcctgct ccccgtgtgt cggacgcgcg tctacaccaa ccactgggca
      121 gtcaaaatcg ccgggggctt cccggaggcc aaccgtatcg ccagcaagta cggattcatc
      181 aacataggac agataggggc cctgaaggac tactaccact tctaccatag caggacgatt
      241 aaaaggtcag ttatctcgag cagagggacc cacagtttca tttcaatgga accaaaggtg
      301 gaatggatcc aacagcaagt ggtaaaaaag cggacaaaga gggattatga cttcagtcgt
      361 gcccagtcta cctatttcaa tgatcccaag tggcccagca tgtggtatat gcactgcagt
      421 gacaatacac atccctgcca gtctgacatg aatatcgaag gagcctggaa gagaggctac
      481 acgggaaaga acattgtggt cactatcctg gatgacggaa ttgagagaac ccatccagat
      541 ctgatgcaaa actacgatgc tctggcaagt tgcgacgtga atgggaatga cttggaccca
      601 atgcctcgtt atgatgcaag caacgagaac aagcatggga ctcgctgtgc tggagaagtg
      661 gcagccgctg caaacaattc gcactgcaca gtcggaattg ctttcaacgc caagatcgga
      721 ggagtgcgaa tgctggacgg agatgtcacg gacatggttg aagcaaaatc agttagcttc
      781 aacccccagc acgtgcacat ttacagcgcc agctggggcc cggatgatga tggcaagact
      841 gtggacggac cagcccccct cacccggcaa gcctttgaaa acggcgttag aatggggcgg
      901 agaggcctcg gctctgtgtt tgtttgggca tctggaaatg gtggaaggag caaagaccac
      961 tgctcctgtg atggctacac caacagcatc tacaccatct ccatcagcag cactgcagaa
     1021 agcggaaaga aaccttggta cctggaagag tgttcatcca cgctggccac aacctacagc
     1081 agcggggagt cctacgataa gaaaatcatc actacagatc tgaggcagcg ttgcacggac
     1141 aaccacactg ggacgtcagc ctcagccccc atggctgcag gcatcattgc gctggccctg
     1201 gaagccaatc cgtttctgac ctggagagac gtacagcatg ttattgtcag gacttcccgt
     1261 gcgggacatt tgaacgctaa tgactggaaa accaatgctg ctggttttaa ggtgagccat
     1321 ctttatggat ttggactgat ggacgcagaa gccatggtga tggaggcaga gaagtggacc
     1381 accgttcccc ggcagcacgt gtgtgtggag agcacagacc gacaaatcaa gacaatccgc
     1441 cctaacagtg cagtgcgctc catctacaaa gcttcaggct gctcggataa ccccaaccgc
     1501 catgtcaact acctggagca cgtcgttgtg cgcatcacca tcaccccccc caggagagga
     1561 gacctggcca tctacctgac ctcgccctct ggaactaggt ctcagctttt ggccaacagg
     1621 ctatttgatc actccatgga aggattcaaa aactgggagt tcatgaccat tcattgctgg
     1681 ggagaaagag ctgctggtga ctgggtcctt gaagtttatg atactccctc tcagctaagg
     1741 aactttaaga ctccaggtaa attgaaagaa tggtctttgg tcctctacgg cacctccgtg
     1801 cagccatatt caccaaccaa tgaatttccg aaagtggaac ggttccgcta tagccgagtt
     1861 gaagacccca cagacgacta tggcacagag gattatgcag gtccctgcga ccctgagtgc
     1921 agtgaggttg gctgtgacgg gccaggacca gaccactgca atgactgttt gcactactac
     1981 tacaagctga aaaacaatac caggatctgt gtctccagct gcccccctgg ccactaccac
     2041 gccgacaaga agcgctgcag gaagtgtgcc cccaactgtg agtcctgctt tgggagccat
     2101 ggtgaccaat gcatgtcctg caaatatgga tactttctga atgaagaaac caacagctgt
     2161 gttactcact gccctgatgg gtcatatcag gataccaaga aaaatctttg ccggaaatgc
     2221 agtgaaaact gcaagacatg tactgaattc cataactgta cagaatgtag ggatgggtta
     2281 agcctgcagg gatcccggtg ctctgtctcc tgtgaagatg gacggtattt caacggccag
     2341 gactgccagc cctgccaccg cttctgcgcc acttgtgctg gggcaggagc tgatgggtgc
     2401 attaactgca cagagggcta cttcatggag gatgggagat gcgtgcagag ctgtagtatc
     2461 agctattact ttgaccactc ttcagagaat ggatacaaat cctgcaaaaa atgtgatatc
     2521 agttgtttga cgtgcaatgg cccaggattc aagaactgta caagctgccc tagtgggtat
     2581 ctcttagact taggaatgtg tcaaatggga gccatttgca aggatgcaac ggaagagtcc
     2641 tgggcggaag gaggcttctg tatgcttgtg aaaaagaaca atctgtgcca acggaaggtt
     2701 cttcaacaac tttgctgcaa aacatgtaca tttcaaggct gc
//
