LOCUS       sequence-158064-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxy-inducible expression of N289A+H290A
            mutant of human CtIP resistant to a siRNA. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-CtIP-siR-N289A-H290A
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             176..202
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg caagcttgcg gccgccgcca ccatgtaccc
      181 ctacgatgtg cctgactacg ccggatccat gaacatctcg ggaagcagct gtggaagccc
      241 taactctgca gatacatcta gtgactttaa ggacctttgg acaaaactaa aagaatgtca
      301 tgatagagaa gtacaaggtt tacaagtaaa agtaaccaag ttgaagcagg aacgaatctt
      361 agatgcacaa agactagaag aattcttcac caaaaatcaa cagctgaggg aacagcagaa
      421 agtccttcat gaaaccatta aagttttaga agatcggtta agagcaggct tatgtgatcg
      481 ctgtgcagta actgaagaac atatgcggaa aaaacagcaa gagtttgaaa atatccggca
      541 gcagaatctt aaacttatta cagaacttat gaatgaaagg aatactctac aggaagaaaa
      601 taaaaagctt tctgaacaac tccagcagaa aattgagaat gatcaacagc atcaagcagc
      661 tgagcttgaa tgtgaggaag acgttattcc agattcaccg ataacagcct tctcattttc
      721 tggcgttaac cggctacgaa gaaaggagaa cccccatgtc cgatacatag aacaa
//
