LOCUS       sequence-158065-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxy-inducible expression of N289A+H290A
            mutant of human CtIP resistant to a siRNA. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-CtIP-siR-N289A-H290A
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gacgcgttta tgtcttctgc tccttgcctt ttggagaaaa tattgcgttg taaggctgac
       61 gtctttttgg acgaggacaa ggatcaagat cttccttaat ataacctctt tccatacaag
      121 tctgagtgga aggaaaacca acttcccaaa aattctctgg tgtgttgggt ggaatgtagc
      181 ggaatcggtg tcttgagcag gaagccaatt tcttttctct ttcttctgct ggcatatctg
      241 cataataaat ttcacattcc ttacacgtgt gcccaagcag ttttcttctc tcctcttttt
      301 tccgaaccac ctcaatatga ggaaaatttt gcaagctagt ctctctttca tcacctttaa
      361 aatacggctc cacaaacgct ttctgcttga ctttgtcttg tttatcacca tgagtgtgta
      421 gtttctttgt ggcagtagac aattcctcct cttcatctgc tgcttgggag aaactgtctg
      481 ccaaacagga ttcatactct tcatgtgttg tccgatcaaa catatcttcc aagctatcat
      541 tcatttttct ttcttcattt gaactttttt ctcccttctg ctcttgtttc ttcattttta
      601 agagaacggt ttcactaacc aatgtacagt ccatgtccac tgtctctcct cctaattttg
      661 actgactgcc atcctttgta tctattacag taacatccat tttatactga gaaaggtctg
      721 ctcccggatc tatactccac tggatatttt caaaggatac atcttggttg ttttgtagag
      781 actttatttt accagttcta catggattta actgaagaac tgatgcaagt tcacatcctc
      841 catg
//
