LOCUS       sequence-158067-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged Rac1 Q61L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-Rac1 Q61L
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    46..70
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        86..195
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             219..923
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(361..379)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggcaaagaat tggatcggac cgaaattaat acgactcact ataggggaat tgtgagcgga
       61 taacaattcc ccggagttaa tccgggacct ttaattcaac ccaacacaat atattatagt
      121 taaataagaa ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac
      181 attttattta caatcaaagg agatatacca tgtctagagt gagcaagggc gaggaggata
      241 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      301 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      361 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      421 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      481 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      541 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      601 tgcgcggcac caacttcccc tccgacggcc ctgtaatgca gaagaagact atgggctggg
      661 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
      721 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
      781 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
      841 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
      901 gcggcatgga cgagctgtac aagggttctg gaagttccat ggtggataac aaattcaata
      961 aagaaaa
//
