LOCUS       sequence-158068-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of H63S
            mutant of human MRE11. Confers resistance to puromycin. Use T-REx 
            cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-MRE11-H63S
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     47..71
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             163..189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga
       61 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
      121 accgatccag cctccggcaa gcttgcggcc gccgccacca tgtaccccta cgatgtgcct
      181 gactacgcca ccggtatgag tactgcagat gcacttgatg atgaaaacac atttaaaata
      241 ttagttgcaa cagatattca tcttggattt atggagaaag atgcagtcag aggaaatgat
      301 acgtttgtaa cactcgatga aattttaaga cttgcccagg aaaatgaagt ggattttatt
      361 ttgttaggtg gtgatctttt tagcgaaaat aagccctcaa ggaaaacatt acatacctgc
      421 ctcgagttat taagaaaata ttgtatgggt gatcggcctg tccagtttga aattctcagt
      481 gatcagtcag tcaactttgg ttttagtaag tttccatggg tgaactatca agatggcaac
      541 ctcaacattt caattccagt gtttagtatt catggcaatc atgacgatcc cacaggggca
      601 gatgcacttt gtgccttgga cattttaagt tgtgctggat ttgtaaatca ctttggacgt
      661 tcaatgtctg tggagaagat agacattagt ccggttttgc ttcaaaaagg aagcacaaag
      721 attgcgctat atggtttagg atccattcca gatgaaaggc tctatcga
//
