LOCUS       sequence-158070-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged Rac1 Q61L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-Rac1 Q61L
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..97)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(104..136)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(join(173..568,569..571,572..751))
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
ORIGIN
        1 ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga
       61 ggttaatcac ttagtgatgg tgatggtgat ggtggtgctc gagatcctcg gggtcttccg
      121 gggcgagttc tggctggctt gcacgtgtat acagctgtgc ggccgcaagc ttttacaaca
      181 gcaggcattt tctcttcctc ttcttcacgg gaggcgggca gaggactgct cggatcgctt
      241 cgtcaaacac tgtcttgagg cctcgctgtg tgagcgccga gcactccagg tattttacgg
      301 caccaatctc cttagccatg gctagaccct gcggataggt gatgggagtc agcttcttct
      361 ccttcagttt ctcgatcgtg tctttatcat ccctaagatc aagtttagtt cccactagga
      421 tgatgggagt gttgggacag tggtgccgca cctcaggata ccactttgca cggacatttt
      481 caaatgatgc aggactcaca agggaaaagc aaattaagaa cacatctgtt tgcggatagg
      541 atagggggcg taatctgtca taatcttcta gtccagctgt atcccataag cccagattca
      601 ccggttttcc atctaccata acattggcag aataattgtc aaagacagta gggatatatt
      661 ctccaggaaa tgcattggtt gtgtaactga tcagtaggca agttttacct acagctccgt
      721 ctcccaccac cacacacttg atggcctgca tggtaccacc agaaccagaa ccaccggatc
      781 cttttggggc ctgggcatcg ttcagttttt tggcttcggc cagcagattg gcgctctgag
      841 atggatcatc aaacaggctc acgatgaagg caaacttctg ctcaacattc aggtttggca
      901 gagaatggat ttccgcaccg gcacgcgtct tttctttatt gaa
//
