LOCUS       sequence-158071-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of H63D
            exonuclease-dead mutant of human MRE11. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-MRE11-H63D
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgcgtctcg agggtacctt atcttctatt tcttcttaaa gaactagtgt tcataaaagg
       61 atcatcatca tcatcctcac ttgattcaaa atcaacccct ttcgatactt gactctggga
      121 catgattttg ctggatgatg tgctggacca cctttgatct gtctttgaag tggtaggaaa
      181 aatgtcttct tccacatctg attcatctac ctcaatcacc tctgaataat tcttagtagt
      241 gacatttcgg gaaggctgct gtcttgtaga tttaaaggca tctataatag acatatttct
      301 agatgctgac acagcagtct ttgagttcct gctacgggta gaagtctcca gaccagtgtc
      361 tgctcttcct ctttgagacc ctcctctcga tgctgaattc tgccctcttc cacctcttcg
      421 acctcttcct cggcctcttc ctttgttggt tgctgctgag atgctatcat cagagtcatt
      481 agccatctgt tctgctaaat ctatactcat aaggtcatca gcactaaagg cagaagcaga
      541 ctcctctgac tgagatctga gtgctctggc cctggtcata gcctcacgga cttcatcatc
      601 ttcttcatta gtattttttt gtctggtttc tctgaaacga cgtacctcct catcgatttt
      661 gtcttcgagg gcatcaatgt gacgttcttt aagaaatcgc tgtgtttttt ccaactggta
      721 tttcactaat tcctcaatgg catctttctc ctccttgtcc acaaattctt gtactgcttc
      781 acccatccct ctttctgtta gcagtgagag ctgcacattc ttctctgcgg tttgaaag
//
