LOCUS       sequence-158073-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to create unc-73B null allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Zheng C, Diaz-Cuadros M, Chalfie M
  TITLE     GEFs and Rac GTPases control directional specificity of neurite 
            extension along the anterior-posterior axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6973-8. doi: 
            10.1073/pnas.1607179113. Epub 2016 Jun 6.
  PUBMED    27274054
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1607179113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: "6973-8"
COMMENT     Sequence Label: TU#1805_CRISPR_unc-73_exon2
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1..23)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(297..372)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cctctgacac atgcagctcc cggagacggt cacagcttgc tctgctaagc ggatgccggg
       61 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
      121 tatgcggcat cagagcagat tgcactgaga gtgcaccata tgcggtgtga aataccgcac
      181 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
      241 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agaaatttca caaaaagcac
      301 cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc
      361 tagctctaaa actcacgaag aacatgcaga acaagacatc tcgcaatagg aggtgtttat
      421 ataggggcaa cacaagaggc ggcagacatc tcgtgagttt gacagagtag tataaagagt
      481 gtgtgtgaga gggtgtaggc atttcataac atattttttg tggaataatt ttggaaaaaa
      541 ggtgtttttt taataatagc ttttataaga gtttctcaaa aattgcatgc tttttttgaa
      601 aaggttgaca aattatagta gtactaaagt tgctatttct agctctaaaa ctcacgaaga
      661 acatgcagaa caagacatct cgcaatagga ggtgtttata taggggcaac acaagaggcg
      721 gcagacatct cgtgagtttg acagagtagt ataaagagtg tgtgtgagag gg
//
