LOCUS       sequence-158079-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to create unc-73E null allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Zheng C, Diaz-Cuadros M, Chalfie M
  TITLE     GEFs and Rac GTPases control directional specificity of neurite 
            extension along the anterior-posterior axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6973-8. doi: 
            10.1073/pnas.1607179113. Epub 2016 Jun 6.
  PUBMED    27274054
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1607179113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: "6973-8"
COMMENT     Sequence Label: TU#1806_CRISPR_unc-73_exon21
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..25)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(297..372)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(393..742)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     748..770
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     762..779
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     763..779
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    787..807
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
       61 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
      121 tatgcggcat cagagcagat tgcactgaga gtgcaccata tgcggtgtga aataccgcac
      181 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
      241 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agaaatttca caaaaagcac
      301 cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc
      361 tagctctaaa actgctggag ttggcgaaga tcaagacatc tcgcaatagg aggtgtttat
      421 ataggggcaa cacaagaggc ggcagacatc tcgtgagttt gacagagtag tataaagagt
      481 gtgtgtgaga gggtgtaggc atttcataac atattttttg tggaataatt ttggaaaaaa
      541 ggtgtttttt taataatagc ttttataaga gtttctcaaa aattgcatgc tttttttgaa
      601 aaggttgaca aattatagta gtactaaagt tgctattttc aggtctcagc agaaaattca
      661 caactaaaaa taaaatttgc aaaacagagt gtgcgccttt aaggtgtaca gttttttatt
      721 cctttattgc tagttttttt tggttttccc agtcacgacg ttgtaaaacg acggccagtg
      781 aattatcaac tatgtataat aaagttgtga gacttttttc ttggcggcac aataa
//
