LOCUS       sequence-158080-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  WT1 targeting donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Bao X, Lian X, Hacker TA, Schmuck EG, Qian T, Bhute VJ, Han T, Shi 
            M, Drowley L, Plowright A, Wang QD, Goumans MJ, Palecek SP
  TITLE     Long-term self-renewing human epicardial cells generated from 
            pluripotent stem cells under defined xeno-free conditions.
  JOURNAL   Nat Biomed Eng. 2016;1. pii: 0003. doi: 10.1038/s41551-016-0003. 
            Epub 2016 Dec 5.
  PUBMED    28462012
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng."; date: "2016"; volume: "1. pii: 0003. doi"; pages: " 
            10.1038/s41551-016-0003. Epub 2016 Dec 5"
COMMENT     Sequence Label: WT1-2A-eGFP PGK PuroR Donor Plasmid
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..69)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cttgctcacc attggcccgg gattctcttc gacatcccct gcttgtttca acagggagaa
       61 gttagtggcg ctagcaagcg ccagctggag tttggtcatg tttctctgat gcatgttgtg
      121 atggcggact aattcatctg accgggcaaa ctttttctga caacttggcc accgacagct
      181 gaagggcttt tcacctgttg acacaattgc cagtcagaga cacttgcaac aaagagacag
      241 gcgcaagttc aactatcaag gcccgagtga agtcatcaca aggcacccac tctgaatttc
      301 ccatcattct gtccctgagc taaccaactg agctcatttc ccaggcagcc ttgaaacagc
      361 agcacctctt ggggctttaa ggatcgaaat attgtaaaaa gttatattct taggtcagaa
      421 aacttggaaa gtgggagata aagaatgagt caagttgggg gaaatgtctg agcaattcat
      481 aattaagggg aaaaacgttt gtttcagtct ctgctgatag aatgttttgt gaactgtgta
      541 aatttctccc ccttgttgag taagttggga actgagatga actgggctgc agaagcctct
      601 gggtcatcta ccctgggtat tactgagtga tcactgtcca gggatctcag ctgtgactga
      661 agcttttaac acttctatct gtgtagatac cctggatggg ctggatactt taaaataaat
      721 cactgatacc agtttacatg ttatgtgttg tttttttttt ttctcttaa
//
