LOCUS       sequence-158081-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  WT1 targeting donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Bao X, Lian X, Hacker TA, Schmuck EG, Qian T, Bhute VJ, Han T, Shi 
            M, Drowley L, Plowright A, Wang QD, Goumans MJ, Palecek SP
  TITLE     Long-term self-renewing human epicardial cells generated from 
            pluripotent stem cells under defined xeno-free conditions.
  JOURNAL   Nat Biomed Eng. 2016;1. pii: 0003. doi: 10.1038/s41551-016-0003. 
            Epub 2016 Dec 5.
  PUBMED    28462012
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng."; date: "2016"; volume: "1. pii: 0003. doi"; pages: " 
            10.1038/s41551-016-0003. Epub 2016 Dec 5"
COMMENT     Sequence Label: WT1-2A-eGFP PGK PuroR Donor Plasmid
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..36
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     28..45
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     29..45
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
       61 tcactatagg gcgaattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga
      121 tatctgcaga attcgccctt ttggccggcc acagtgatgc aagagccaga tgcaaaataa
      181 gaggggcctc tggacctgac ttttaggctg cccaaacttt gagctgcact tcaatgtaaa
      241 acagtctcca tagctaggct tccatctgtt tgagacgcat tttaaatgaa gaaataaaac
      301 ccggaatgca tgcaagcccc aagataatgg agctgagagg ggctgcacca aattaatctc
      361 cttatttaca attcaaaaac catacaccag aacagaaaga gagtttatag tttatagata
      421 atatagatct ttgctttcac actcagttgc cctggaaagg ctgagcagtt ttcaatctgc
      481 tgagctgatt tatagcaagt acagctgtaa attcaattgt actaggtgtt cagcctatgc
      541 cctcccagtg atgtatctga acttttcaaa aggactagtt gtcattaaca tctctctatt
      601 ttcttttgct gatctgggat gatgggatgc aatgacaatc tgcttaaaat aaaccttaaa
      661 accttaaaac ataatcacca catcatccac aaaccccatt ca
//
