LOCUS       sequence-158085-        7299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express unc-53a cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Zheng C, Diaz-Cuadros M, Chalfie M
  TITLE     GEFs and Rac GTPases control directional specificity of neurite 
            extension along the anterior-posterior axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6973-8. doi: 
            10.1073/pnas.1607179113. Epub 2016 Jun 6.
  PUBMED    27274054
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1607179113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: "6973-8"
COMMENT     Sequence Label: TU#1450_unc-53a_cDNA
FEATURES             Location/Qualifiers
     source          1..7299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5424..5523)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5540..5559)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5541..5559)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5564..5580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5564..5580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5682..5701)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5693..6502
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5760..5779)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6649..7237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7138..7157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta atgacgacgt caaatgtaga attgatacca atctacacgg attgggccaa
      721 tcggcacctt tcgaagggca gcttatcaaa gtcgattagg gatatttcca atgattttcg
      781 cgactatcga ctggtttctc agcttattaa tgtgatcgtt ccgatcaacg aattctcgcc
      841 tgcattcacg aaacgtttgg caaaaatcac atcgaacctg gatggcctcg aaacgtgtct
      901 cgactacctg aaaaatctgg gtctcgactg ctcgaaactc accaaaaccg atatcgacag
      961 cggaaacttg ggtgcagttc tccagctgct cttcctgctc tccacctaca agcagaagct
     1021 tcggcaactg aaaaaagatc agaagaaatt ggagcaacta cccacatcca ttatgccacc
     1081 cgcggtttct aaattaccct cgccacgtgt cgccacgtca gcaaccgctt cagcaactaa
     1141 cccaaattcc aactttccac aaatgtcaac atccaggctt cagactccac agtcaagaat
     1201 atcgaaaatt gattcatcaa agattggtat caagccaaag acgtctggac ttaaaccacc
     1261 ctcatcatca accacttcat caaataatac aaattcattc cgtccgtcga gccgttcgag
     1321 tggcaataat aatgttggct cgacgatatc cacatctgcg aagagcttag aatcatcatc
     1381 aacgtacagc tctatttcga atctaaaccg acctacctcc caactccaaa aaccttctag
     1441 accacaaacc cagctagttc gtgttgctac aactacaaaa atcggaagct caaagctagc
     1501 cgctccgaaa gccgtgagca ccccaaaact tgcttctgtg aagactattg gagcaaaaca
     1561 agagcccgat aacagcggtg gtggtggtgg tggaatgctg aaattaaagt tattcagtag
     1621 caaaaaccca tcttcctcat cgaatagccc acaacctacg agaaaggcgg cggcggtgcc
     1681 tcaacaacaa actttgtcga aaatcgctgc cccagtgaaa agtggcctga agccgccgac
     1741 cagtaagctg ggaagtgcca cgtctatgtc gaagctttgt acgccaaaag tttcctaccg
     1801 taaaacggac gccccaatca tatctcaaca agactcgaaa cgatgctcaa agagcagtga
     1861 agaagagtcc ggatacgctg gattcaacag cacgtcgcca acgtcatcat cgacggaagg
     1921 ttccctaagc atgcattcca catcttccaa gagttcaacg tcagacgaaa agtctccgtc
     1981 atcagacgat cttactctta acgcctccat cgtgacagct atcagacagc cgatagccgc
     2041 aacaccggtt tctccaaata ttatcaacaa gcctgttgag gaaaaaccaa cactggcagt
     2101 gaaaggagtg aaaagcacag cgaaaaaaga tccacctcca gctgttccgc cacgtgacac
     2161 ccagccaaca atcggagttg ttagtccaat tatggcacat aagaagttga caaatgaccc
     2221 cgtgatatct gaaaaaccag aacctgaaaa gctccaatca atgagcatcg acacgacgga
     2281 cgttccaccg cttccacctc taaaatcagt tgttccactt aaaatgactt caatccgaca
     2341 accaccaacg tacgatgttc ttctaaaaca aggaaaaatc acatcgcctg tcaagtcgtt
     2401 tggatatgag cagtcgtccg cgtctgaaga ctccattgtg gctcatgcgt cggctcaggt
     2461 gactccgccg acaaaaactt ctggtaatca ttcgctggag agaaggatgg gaaagaataa
     2521 gacatcagaa tccagcggct acacctctga cgccggtgtt gcgatgtgcg ccaaaatgag
     2581 ggagaagctg aaagaatacg atgacatgac tcgtcgagca cagaacggct atcctgacaa
     2641 cttcgaagac agttcctcct tgtcgtctgg aatatccgat aacaacgagc tcgacgacat
     2701 atccacggac gatttgtccg gagtagacat ggcaacagtc gcctccaaac atagcgacta
     2761 ttcccacttt gttcgccatc ccacgtcttc ttcctcaaag ccccgagtcc ccagtcggtc
     2821 ctccacatca gtcgattctc gatctcgagc agaacaggag aatgtgtaca aacttctgtc
     2881 ccagtgccga acgagccaac gtggcgccgc tgccacctca accttcggac aacattcgct
     2941 aagatccccg ggatactcat cctattctcc acacttatca gtgtcagctg ataaggacac
     3001 aatgtctatg cactcacaga ctagtcgacg accttcttca caaaaaccaa gctattcagg
     3061 ccaatttcat tcacttgatc gtaaatgcca ccttcaagag ttcacatcca ccgagcacag
     3121 aatggcggct ctcttgagcc cgagacgggt gccgaactcg atgtcgaaat atgattcttc
     3181 aggatcctac tcggcgcgtt cccgaggtgg aagctctact ggtatctatg gagagacgtt
     3241 ccaactgcac agactatccg atgaaaaatc ccccgcacat tctgccaaaa gtgagatggg
     3301 atcccaacta tcactggcta gcacgacagc atatggatct ctcaatgaga agtacgaaca
     3361 tgctattcgg gacatggcac gtgacttgga gtgttacaag aacactgtcg actcactaac
     3421 caagaaacag gagaactatg gagcattgtt tgatcttttt gagcaaaagc ttagaaaact
     3481 cactcaacac attgatcgat ccaacttgaa gcctgaagag gcaatacgat tcaggcagga
     3541 cattgctcat ttgagggata ttagcaatca tcttgcatcc aactcagctc atgctaacga
     3601 aggcgctggt gagcttcttc gtcaaccatc tctggaatca gttgcatccc atcgatcatc
     3661 gatgtcatcg tcgtcgaaaa gcagcaagca ggagaagatc agcttgagct cgtttggcaa
     3721 gaacaagaag agctggatcc gctcctcact ctccaagttc accaagaaga agaacaagaa
     3781 ctacgacgaa gcacatatgc catcaatttc cggatctcaa ggaactcttg acaacattga
     3841 tgtgattgag ttgaagcaag agctcaaaga acgcgatagt gcactttacg aagtccgcct
     3901 tgacaatctg gatcgtgccc gcgaagttga tgttctgagg gagacagtga acaagttgaa
     3961 aaccgagaac aagcaattaa agaaagaagt ggacaaactc accaacggtc cagccactcg
     4021 tgcttcttcc cgcgcctcaa ttccagttat ctacgacgat gagcatgtct atgatgcagc
     4081 gtgtagcagt acatcagcta gtcaatcttc gaaacgatcc tctggctgca actcaatcaa
     4141 ggttactgta aacgtggaca tcgctggaga aatcagttcg atcgttaacc cggacaaaga
     4201 gataatcgta ggatatcttg ccatgccaac cagtcagtca tgctggaaag acattgatgt
     4261 ttctattcta ggactatttg aagtctacct atccagaatt gatgtggagc atcaacttgg
     4321 aatcgatgct cgtgattcta tccttggcta tcaaattggt gaacttcgac gcgtcattgg
     4381 agactccaca accatgataa ccagccatcc aactgacatt cttacttcct caactacaat
     4441 ccgaatgttc atgcacggtg ccgcacagag tcgcgtagac agtctggtcc ttgatatgct
     4501 tcttccaaag caaatgattc tccaactcgt caagtcaatt ttgacagaga gacgtctggt
     4561 gttagctgga gcaactggaa ttggaaagag caaactggcg aagaccctgg ctgcttatgt
     4621 atctattcga acaaatcaat ccgaagatag tattgttaat atcagcattc ctgaaaacaa
     4681 taaagaagaa ttgcttcaag tggaacgacg cctggaaaag atcttgagaa gcaaagaatc
     4741 atgcatcgta attctagata atatcccaaa gaatcgaatt gcatttgttg tatccgtttt
     4801 tgcaaatgtc ccacttcaaa acaacgaagg tccatttgta gtatgcacag tcaaccgata
     4861 tcaaatccct gagcttcaaa ttcaccacaa tttcaaaatg tcagtaatgt cgaatcgtct
     4921 cgaaggattc atcctacgtt acctccgacg acgggcggta gaggatgagt atcgtctaac
     4981 tgtacagatg ccatcagagc tcttcaaaat cattgacttc ttcccaatag ctcttcaggc
     5041 cgtcaataat tttattgaga aaacgaattc tgttgatgtg acagttggtc caagagcatg
     5101 cttgaactgt cctctaactg tcgatggatc ccgtgaatgg ttcattcgat tgtggaatga
     5161 gaacttcatt ccatatttgg aacgtgttgc tagagatggc aaaaaaacct tcggtcgctg
     5221 cacttccttc gaggatccca ccgacatcgt ctctgaaaaa tggccgtggt tcgatggtga
     5281 aaacccggag aatgtgctca aacgtcttca actccaagac ctcgtcccgt cacctgccaa
     5341 ctcatcccga caacacttca atcccctcga gtcgttgatc caattgcatg ctaccaagca
     5401 tcagaccatc gacaacattt gacacccagc tttcttgtac aaagttggca ttataagaaa
     5461 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     5521 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     5581 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaataatatc
     5641 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     5701 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     5761 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     5821 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     5881 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     5941 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     6001 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     6061 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     6121 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     6181 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     6241 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     6301 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     6361 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     6421 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     6481 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     6541 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     6601 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6661 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6721 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6781 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6841 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6901 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6961 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7021 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7081 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7141 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7201 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7261 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
