LOCUS       sequence-158087-        7464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express unc-73b cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Zheng C, Diaz-Cuadros M, Chalfie M
  TITLE     GEFs and Rac GTPases control directional specificity of neurite 
            extension along the anterior-posterior axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6973-8. doi: 
            10.1073/pnas.1607179113. Epub 2016 Jun 6.
  PUBMED    27274054
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1607179113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: "6973-8"
COMMENT     Sequence Label: TU#1448_unc-73b_cDNA
FEATURES             Location/Qualifiers
     source          1..7464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5589..5688)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5705..5724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5706..5724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5729..5745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5729..5745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5847..5866)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5858..6667
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5925..5944)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6814..7402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7303..7322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta atgggcgatt atgatggaaa aatgacgatg aaagcagaag acattctgca
      721 tgttcttcgt gacggcattg cagtacttcc tggaggacga tgtagagccg gacaagcagt
      781 tatcgtatgt ccatcacgag agcaacctgt caatcaggat aatttacgca atgtctttct
      841 gtatttattt gaagttactt cgaagatggc acgcgaaaag ggattccttg tggtaattga
      901 tatgcgtgga aaacaaacat ggaccaatgt tcgacacatt ctgaaagctc tcagtagtat
      961 tgaaagctca tcaacagtac aagtgttcat catcaagccc gaaaaattct gggagaaaca
     1021 aaaagcacaa atgtcccttg gaacgtggga ttttgaagtt gaaatgatct cattcgaatc
     1081 gcttatcaaa ataatcgact cgtcacattt accgaaaacc gtcggcggaa gctatccata
     1141 tgatcatgat gaatggcttg agctcaggtt ggatctcgag aaatggatct ggaatatcac
     1201 tgaaattatg gaaaagctcg agtctgtacg tcgagaaatt tgcgaaggcg agcagcctgt
     1261 cgatgtgaca actgccaacg ctgcgctgaa aaagagccaa cacgcaaaga atagtatatt
     1321 taatgttccg gtggaaggaa ttgagacaga agggaataaa attgcaactc gaatattaaa
     1381 accgtcgaag ggtgtcaaaa acccggacct tgaagcgaca accccatata tttcaaattt
     1441 gaccgattcg ctcaggttgc tcaaaggcga agtagccaag aattgggaag ttcgacagat
     1501 ggaactttca aaagtgtacc agcagaaaca atttgaacgt gacgcagaac atatgattga
     1561 gacgcttcgg ccgtacaaaa aagcctgtga acgtagtatg ggtgatgttg gtggttgtgc
     1621 aaacgatgtc gttcggctct ctgcggagtt tgagcagttc cagattgctg tgagaggtat
     1681 ggaagtgtct gtgaaacaag ttttcgacaa ggaaaatcat ttgagaacta ttggagcccg
     1741 gaatcagatt accgatcacg tggcggcacg acttgccgaa gaatggcacc tagtcaaaga
     1801 actcatggag aggcggagtg cagttttgca caatgccaaa gaattcttta cgtcagctca
     1861 acgatatttc gcagaagtac ccagatggac tgcacaaccc ggagtaaatc caaacgatgt
     1921 gctcttcaat caggagtcgt tggaagaagc cattcgaaaa catgacgcat tttgggctca
     1981 agttgaagag gtttatgcgc aagcttatga tgatgcgtca aaagtgacaa gagctctgaa
     2041 agaagctgat gctgaagata atgttgctag ggagcattcg agtagactgc aaagggcaca
     2101 taagcagttg atggaaaagt ggaaagaaag acaggttctg ctccatcata tgcttgcaat
     2161 gattgcgttt gaaaccgacg tgaggttagt cgtcgattgg ctcgaacaac acggtgaacc
     2221 gtatcttcga cgtaacattc atattggtga aaacgtgaat caggcgagaa gcttccagag
     2281 gaatcacaca aattttcaac gggttgctgc gaatacctat ggtaacgtgc aaaagcttca
     2341 tcaagtctat caggatgtga ctaaatctgg cagtaaaatc tgcgacgttg acactatgca
     2401 cggtctcatg acggatctat cggcaaaaat tgagaagttc acgaagatcg agaactctcg
     2461 ggagctgctg ctacggcaat cggttctttt tcacactcac tataaagagt tgacggattg
     2521 gtatggaaaa atgggcgaga agtataagga tcggaggata gatctgacgg ttccaacttg
     2581 tgataagaat aaggaacgat ttgtgttgga gactgacgag actgctcaag catacgccat
     2641 gacaatagac gaagggaaga cgctgatcga aatgatgcag agagctacac gggtttttga
     2701 tgttgattac tcggctagtg ttgcacacat tcaactgctt atctcagata ttgaagacaa
     2761 gaacacacag atctcatcag aatggtctcc acgacgaact ctcctacaca tcgcctcaaa
     2821 gtttgccatg ttcgaacaga atcagtttga agtgttggaa cagatcaaaa gttgggaagt
     2881 tgatatgcga gaaatggcag aatctgactc attttacgac aaggctgatt cggttttacc
     2941 attccataaa gataaccaac aacaagttcg caacgcgatc gcggacattc aaaaatctgc
     3001 aaaagagcta tcgcaagctt tgtatagtca aaagttgacg gatcttcggg acaaacggga
     3061 tagagtggtg ctcgaagcaa tacgagagca tgttcgagaa cttgaaattg ccgaatcccg
     3121 agtgatgacg tacgcgaatg aaacttcaat gagaatgcaa gcagcttcgg aagtcggtga
     3181 tctcagaagg atatccaata gtgtttgtaa gcttattgat aatcaattat cagccctcac
     3241 gcagctagga gtcattccaa atgactataa tgatactgtg caaaagcaag aagagttgag
     3301 agcttttcgt gatgcagttc aacataagct caaggaaccg tatgacgcat ttgttataag
     3361 atttcgagag ctgatggaaa atcggctggc gaatcgagat gaagtagtct accacaatga
     3421 agcaattcaa gctaaatact gtcgactcat gaatctttgt gaagatcgga ataagttact
     3481 aaaatccgca catggatgct acaagaccta tgagacagca gtgcttccaa tactcaatca
     3541 gttggagtct gaatatcact ctccgacggt tacggattgg tgtgcaggat gtacatcttc
     3601 gatcgacgcc gatcgagcag cttatgtggc agatctgctc agcaagcaca tggactataa
     3661 agaacgattc ggtaaaggat gtacgtatgc gctgagaaac ggtgactttc tgctacgtta
     3721 tattcgaaga tcgacggtga accaaagtga acggaagcga catgaaacga agattgctga
     3781 catgaagaac aatattcgag aacgacaatc gaatattctc gagctatgga tgcagaaaaa
     3841 attgttgttg gaaggatgtc agtccttcat tttcatagaa gccagtgcga aagaactttt
     3901 ggaatttatg aaaggcgaag gtaatcgaaa gttgaagcat tttgagaaga agggtcgtcg
     3961 agagtcaacc gatgatgacg atgaattcgc aaagttcaaa tctgaagtga agcagaagaa
     4021 gacggatatt cagacgttct taatgctgag cacaaatgaa actatgagcc gaggggttca
     4081 cggagacgaa attgataggt gtattgagca ggtgaaagat gagttcagtc gattctcacg
     4141 acgtgttggg gactgtgaag ttgtattgag aggggaaaat ggatcaacgc agtcgtcgaa
     4201 agacgaattc tcacttgatc gacactcgga tactgccata ttcaatgaga agaagatcaa
     4261 tgaacgacgt gaggagaatc gaaagatgct tgagccaatg cgagagctta ttcaatccga
     4321 acgggattat atcaaagatc tcgagagatg tgttaacata tacgtcaagg aatttgatca
     4381 agctgcgaag aatggaacaa ttccgacgtt gaatccgctg aaatatgaaa ttttcggaaa
     4441 cattgagaag attttcaagt ttcataatga caaacttctt catgagctta tcaagtatga
     4501 gaatcaacca gaagctgttg gtgcatcgtt tatagtttgg attgatttgc tcaatgagct
     4561 ctacacggaa tattgcgtga acaaggagca gaagaaccat gtcatcgcga cacccgacgc
     4621 cgtttcgttt ttcaccggta tccgagaacg acacggattg gagatcaata acgagattgc
     4681 gagtctcttg ataaagcctg ttcagagaat aacacgctat cgtctgttga ttgagcaatt
     4741 gatgagaagt tgtacggata agacgaatga tctcaaagag gcgtacgaag tggtgtgcag
     4801 tgtgcctaga aaagtgaacg atctcattca ttacaactgt ttggatttga aggatttcaa
     4861 ggttgatgag cttggaccat ttgtcactca agacactttg acgttctggg agccacgagc
     4921 ctactttaag ggacgaggaa aagagcgtca agtattctta ttcgatattt cgattgtttt
     4981 cgctaaacgc attgaagttt caccgaaaaa tatcaaatat gtgatcaaag gaaagcccct
     5041 tccactaagc gaagtgtcaa ttgtggagca cgtcgaaggc gacacttgcc gattcggtct
     5101 tcgtgttgga actgtgtcgt cgaacgataa tcgcattgat ttgaaggcca acaatcatca
     5161 tacaaaagtg aaatgggttc agaaaatccg tgatctaaca gctggaatgt tgccattggg
     5221 tcttggagta tcggaggctt actcagttgg aacactatcg acagcccgta gtgtctcagt
     5281 gcgatcaggt gcatccacgt cgtctggtgg tgagaatcga caatcacagg atgtcgagtc
     5341 acttttacgc catcgataca gtgttcacag ttgtgatagt gagcagtcat cggaggtctg
     5401 gatcgtgacg gcagattttg atggacaagt cgaagggcat ttgactgtgc ataaagggga
     5461 tcgagttgag attgtggaag accaagccac cgattgtgcc gaatacgtgc aagtggtact
     5521 gtgtgatcag ccaacgaaac acggactcgt acccgcctcc ataattgctc cacaaaacag
     5581 cgagtaacac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     5641 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     5701 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     5761 gtctcaaaat ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa
     5821 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     5881 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     5941 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     6001 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     6061 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     6121 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     6181 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     6241 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     6301 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     6361 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     6421 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     6481 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     6541 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     6601 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     6661 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     6721 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     6781 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6841 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6901 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6961 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7021 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7081 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7141 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7201 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7261 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7321 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7381 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7441 ttgctggcct tttgctcaca tgtt
//
