LOCUS       sequence-158089-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged Tiam1 Dh/PH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-TIAM1 DH/PH
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             281..457
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
ORIGIN
        1 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
       61 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
      121 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
      181 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
      241 cggcggcatg gacgagctgt acaagggttc tggaagttcc atggtggata acaaattcaa
      301 taaagaaaag acgcgtgccg gtgcggaaat ccattctctg ccaaacctga atgttgagca
      361 gaagtttgcc ttcatcgtga gcctgtttga tgatccatct cagagcgcca atctgctggc
      421 cgaagccaaa aaactgaacg atgcccaggc cccaaaagga tccggtggtt ctggttctgg
      481 tggtaccggc gcgccggcga ccatgagaca actctcggat gcagataagc tgcgcaaggt
      541 gatctgcgag ctcctggaga cggagcgcac ctacgtgaag gatttaaact gtcttatgga
      601 gagataccta aagcctcttc aaaaagaaac ttttctcacc caggatgagc ttgacgtgct
      661 ttttggaaat ttaacggaaa tggtagagtt tcaagtagaa ttccttaaaa ctctagaaga
      721 tggagtgaga ctggtacctg atttggaaaa gcttgagaag gttgatcaat ttaagaaagt
      781 gctgttctct ctggggggat cattcctgta ttatgctgac cgcttcaagc tctacagtgc
      841 cttctgcgcc agccacacaa aagttcccaa ggtcctggtg aaagccaaga cagacacggc
      901 tttcaaggca ttcttggatg cccagaaccc gaagcagcag cactcatcca cgctggagtc
      961 gtacctcatc aagcccatcc agaggatcct caagtaccca cttctgctca gggagctgtt
     1021 cg
//
