LOCUS       sequence-158090-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged Tiam1 Dh/PH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-TIAM1 DH/PH
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     83..102
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(192..215)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(222..254)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
ORIGIN
        1 caacacacta ttgcaatgaa aataaatttc ctttattagc cagaagtcag atgctcaagg
       61 ggcttcatga tgtccccata atttttggca gagggaaaaa gatcgatctc agtggtattt
      121 gtgagccagg gcattggcca caccagccac caccttctga taggcagcct gcacctgagg
      181 ttaatcactt agtgatggtg atggtgatgg tggtgctcga gatcctcggg gtcttccggg
      241 gcgagttctg gctggcttgc acgtgtatac agctgtgcgg ccgcttactc ggttttgagg
      301 agctgtcttc tgtgcttatc acgcaggatt gaatgcacag cctttaggaa atcctttcgg
      361 ctctctgggg agctgcagca caagtgaaag accctctccg gcctcccttc agactcggat
      421 tttacatgga caatttcaca cacggcattt gcctctgcat ctgcactcgc caaagctcga
      481 acctgcagcg cttccgtggg gatcatgtgt cgaaatctga aggggtccca gtcctcataa
      541 atggaaagcc tgtgagatcc tacaagtttc ttcttctgtt tggaaccatc tttatacaca
      601 aggaccacag cagttttgaa gacgaatgct gccaactctg gttccttttt ccacttgccc
      661 agcgaggccg gcgggttcag ccagatcacg gtagtgtgca aaagcaggtc tcccatgctc
      721 agatctgcaa cctctttttt ctcaccagtc tgttcagcaa tcagctggtc aaacacagcc
      781 ccaaactctt catggatttt ctgcatctca ttgatgtgac tggcaacctt gttcatggtc
      841 ttgatggcca cgtccaggtg gtagtgctcc tcgctctccg catcggtcag ggcgaacagc
      901 tccctgagca gaagtgggta cttgaggatc ctctggatgg gcttgatgag gtacgactcc
      961 agcgtggatg agtgctgctg cttcgggttc tgggcatcca a
//
