LOCUS       sequence-158155-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxy-inducible expression of mutant 
            human Ku70 unable to bind DNA and resistant to siRNA. Confers 
            resistance to puro. Use T-REx cells for doxycycline-inducible 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Lamaa A, Le Bras M, Skuli N, Britton S, Frit P, Calsou P, Prats H, 
            Cammas A, Millevoi S
  TITLE     A novel cytoprotective function for the DNA repair protein Ku in 
            regulating p53 mRNA translation and function.
  JOURNAL   EMBO Rep. 2016 Apr;17(4):508-18. doi: 10.15252/embr.201541181. Epub 
            2016 Mar 10.
  PUBMED    26964895
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embr.201541181"; journalName: "EMBO Rep"; date: "2016-04";
            volume: "17"; issue: "4"; pages: "508-18"
COMMENT     Sequence Label: pICE-HA-Ku70-siR-Mut6E
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             174..200
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg caagcttacc ggtcgccacc atgtacccct
      181 acgatgtgcc tgactacgcc ctcgagtcag ggtgggagtc atattacaaa accgagggcg
      241 atgaagaagc agaggaagaa caagaagaga accttgaagc aagtggagac tataaatatt
      301 caggaagaga tagtttgatt tttttggttg atgcctccaa ggctatgttt gaatctcaat
      361 ccgaggacga actcacccct tttgacatga gcatccagtg tatccaaagt gtgtacatca
      421 gtaagatcat aagcagtgat cgagatctct tggctgtggt gttctatggt accgagaaag
      481 acaaaaattc agtgaatttt aaaaatattt acgtcttaca ggagctggat aatccaggtg
      541 caaaacgaat tctagagctt gaccagttta aggggcagca gggacaaaaa cgtttccaag
      601 acatgatggg ccacggatct gactactcac tcagtgaagt gctgtgggtc tgtgccaacc
      661 tctttagtga tgtccaattc aagatgagtc ataagaggat catgctgttc accaatgaag
      721 acaaccccca tggcaatgac agtgccaaag ccagccgggc caggaccaaa gccggtgatc
      781 tcc
//
