LOCUS       sequence-158157-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of 
            wild-type human Ku70 resistant to a siRNA. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Lamaa A, Le Bras M, Skuli N, Britton S, Frit P, Calsou P, Prats H, 
            Cammas A, Millevoi S
  TITLE     A novel cytoprotective function for the DNA repair protein Ku in 
            regulating p53 mRNA translation and function.
  JOURNAL   EMBO Rep. 2016 Apr;17(4):508-18. doi: 10.15252/embr.201541181. Epub 
            2016 Mar 10.
  PUBMED    26964895
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embr.201541181"; journalName: "EMBO Rep"; date: "2016-04";
            volume: "17"; issue: "4"; pages: "508-18"
COMMENT     Sequence Label: pICE-HA-Ku70-siR-WT
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     47..71
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             161..187
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga
       61 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
      121 accgatccag cctccggcaa gcttaccggt cgccaccatg tacccctacg atgtgcctga
      181 ctacgccctc gagtcagggt gggagtcata ttacaaaacc gagggcgatg aagaagcaga
      241 ggaagaacaa gaagagaacc ttgaagcaag tggagactat aaatattcag gaagagatag
      301 tttgattttt ttggttgatg cctccaaggc tatgtttgaa tctcaatccg aggacgaact
      361 cacccctttt gacatgagca tccagtgtat ccaaagtgtg tacatcagta agatcataag
      421 cagtgatcga gatctcttgg ctgtggtgtt ctatggtacc gagaaagaca aaaattcagt
      481 gaattttaaa aatatttacg tcttacagga gctggataat ccaggtgcaa aacgaattct
      541 agagcttgac cagtttaagg ggcagcaggg acaaaaacgt ttccaagaca tgatgggcca
      601 cggatctgac tactcactca gtgaagtgct gtgggtctgt gccaacctct ttagtgatgt
      661 ccaattcaag atgagtcata agaggatcat gctgttcacc aatgaagaca acccccatgg
      721 caatgacagt gccaaagcca gccgggccag gaccaaagcc ggtgatctcc gagatacagg
      781 catcttcctt gacttgatgc acctgaagaa
//
