LOCUS       sequence-158161-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human LIG3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Weber AR, Krawczyk C, Robertson AB, Kusnierczyk A, Vagbo CB, 
            Schuermann D, Klungland A, Schar P
  TITLE     Biochemical reconstitution of TET1-TDG-BER-dependent active DNA 
            demethylation reveals a highly coordinated mechanism.
  JOURNAL   Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806.
  PUBMED    26932196
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-2"; volume: "7:10806. doi"; pages: " 
            10.1038/ncomms10806"
COMMENT     Sequence Label: pGEX4T-hLIG3
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             44..70
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      624..633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atcggatctg gttccgcgtg gatccccgaa ttcccgggtc gactacccat acgatgttcc
       61 agattacgct cttatggctg agcaacggtt ctgtgtggac tatgccaagc gtggcacagc
      121 tggctgcaaa aaatgcaagg aaaagattgt gaagggcgta tgccgaattg gcaaagtggt
      181 gcccaatccc ttctcagagt ctgggggtga tatgaaagag tggtaccaca ttaaatgcat
      241 gtttgagaaa ctagagcggg cccgggccac cacaaaaaaa atcgaggacc tcacagagct
      301 ggaaggctgg gaagagctgg aagataatga gaaggaacag ataacccagc acattgcaga
      361 tctgtcttct aaggcagcag gtacaccaaa gaagaaagct gttgtccagg ctaagttgac
      421 aaccactggc caggtgactt ctccagtgaa aggcgcctca tttgtcacca gtaccaatcc
      481 ccggaaattt tctggctttt cagccaagcc caacaactct ggggaagccc cctcgagccc
      541 cacccctaag agaagtctgt cttcaagcaa atgtgacccc aggcataagg actgtctgct
      601 acgggagttt cgaaagttat gcgccatggt ggccgataat cctagctaca acacgaagac
      661 ccagatcatc caggacttcc ttcggaaagg ctcagcagga gatggtttcc acggtgatgt
      721 gtacctaaca gtgaagctgc tgctgccagg agtcat
//
