LOCUS       sequence-158165-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             281..457
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
ORIGIN
        1 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
       61 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
      121 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
      181 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
      241 cggcggcatg gacgagctgt acaagggttc tggaagttcc atggtggata acaaattcaa
      301 taaagaaaag acgcgtgccg gtgcggaaat ccattctctg ccaaacctga atgttgagca
      361 gaagtttgcc ttcatcgtga gcctgtttga tgatccatct cagagcgcca atctgctggc
      421 cgaagccaaa aaactgaacg atgcccaggc cccaaaagga tccggatctg gtggatctgg
      481 taaaatgggc atgactgagg acgacaagag aagctgctgc ttgttagaga ttcaggagac
      541 cgaggccaag tactaccgca ccctggagga cattgagaag aactacatgg gtcccttgcg
      601 gctggtgctg agcccggcgg atatggctgc tgtcttcatc aacctggagg acctcatcaa
      661 ggtgcatcac agctttctgc gagccatcga tgtgtccatg atggctggtg gcagtaccct
      721 ggctaaggtc tttctggagt ttaaggaaag gctcctgatc tatggagagt actgtagcca
      781 catggaacac gctcagagta cactgaacca gctcctcgcc agccgagagg acttcaggca
      841 gaaagtggag gagtgcacac tcagggttca ggatggcaag ttcaagctgc aagacctgct
      901 ggtggtgccc atgcaacggg tgctgaagta ccacctgctg ctcaaggagc tcctgagcca
      961 ttctgcagac cgaccagaa
//
