LOCUS       sequence-158166-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  serotonin receptor minigene with consensus splice sites that 
            expresses the full length serotonin receptor with a C-terminal EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Zhang Z, Shen M, Gresch PJ, Ghamari-Langroudi M, Rabchevsky AG, 
            Emeson RB, Stamm S
  TITLE     Oligonucleotide-induced alternative splicing of serotonin 2C 
            receptor reduces food intake.
  JOURNAL   EMBO Mol Med. 2016 Aug 1;8(8):878-94. doi: 10.15252/emmm.201506030.
  PUBMED    27406820
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.15252/emmm"; 
            journalName: "EMBO Mol Med"; date: "2016-08-1- 1"; volume: "8"; 
            issue: "8"; pages: "878-94"
COMMENT     Sequence Label: FRT_htr2c(c)-GFP
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag ccaccatggt gaacctgagg aatgcggtgc
      121 attcattcct tgtgcaccta attggcctat tggtttggca atgtgatatt tctgtgagcc
      181 cagtagcagc tatagtaact gacattttca atacctccga tggtggacgc ttcaaattcc
      241 cagacggggt acaaaactgg ccagcacttt caatcgtcat cataataatc atgacaatag
      301 gtggcaacat ccttgtgatc atggcagtaa gcatggaaaa gaaactgcac aatgccacca
      361 attacttctt aatgtcccta gccattgctg atatgctagt gggactactt gtcatgcccc
      421 tgtctctcct ggcaatcctt tatgattatg tctggccact acctagatat ttgtgccccg
      481 tctggatttc tttagatgtt ttattttcaa cagcgtccat catgcacctc tgcgctatat
      541 cgctggatcg gtatgtagca atacgtaatc ctattgagca tagccgtttc aattcgcgga
      601 ctaaggccat catgaagatt gctattgttt gggcaatttc tacaggtaag taaaactttt
      661 tggccataag aattgcagcg gctatgctca atactttcgg attatgtact gtgaacaacg
      721 tacagacgtc gactggtaac atttgcgttt gatcgggttc ttttatttag taattattct
      781 taacctattt atctgatatt taggttaaca ataatgaa
//
