LOCUS       sequence-158168-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..72)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(79..111)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
ORIGIN
        1 cagccaccac cttctgatag gcagcctgca cctgaggtta atcacttagt gatggtgatg
       61 gtgatggtgg tgctcgagat cctcggggtc ttccggggcg agttctggct ggcttgcacg
      121 tgtatacagc tgtgcggccg caagctttta tgctccagga gcatccacat ctgcaggtga
      181 actcatcttg caggggggga tcacctccag gcattccttg tgtgccccga cgccacatct
      241 ggtacacagg tatccctggt agaaggtacc cctgagaaac atcttgcagg ctttgcagtt
      301 ggtagtcttg tcgaatgtgt acatctggaa gctatgatgg ttggcattgg ccttatctgg
      361 cttgatgttt gacatggcca tctcgaactg ctccatccac ttccgcttca tgtcttccgt
      421 cttgcagaag aactgaaagc cttgctttcc ttggaggtga atcaggtaga agccatagga
      481 ccacttcttg atgtccttgt tgtgcatcgg gtcatcggtc atcttgtgga agagcagctc
      541 aatgacctcc ttcagctcat agctgtagcc cttcctcttg cacacgatga ccaccttgtc
      601 aaataggaac aggtacctgt cttgcttggt gtggttgact atggaccgga ctttaagctc
      661 cccgtcaatc tttggcctcc caaattcctc cagcttcact tgcaggtttt ctatggagca
      721 ctggaactcg ctaatcttct tcaaggtctc cttgtcccgc ttcacttcat tgatgtacat
      781 ggccaagtcc tgcatggctt ccagggcttc tttgagctgt tgtctttctg gtcggtctgc
      841 agaatggctc aggagctcct tgagcagcag gtggtacttc agcacccgtt gcatgggcac
      901 caccagcag
//
