LOCUS       sequence-158174-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 
            4-713).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Chen R, Rato C, Yan Y, Crespillo-Casado A, Clarke HJ, Harding HP, 
            Marciniak SJ, Read RJ, Ron D
  TITLE     G-actin provides substrate-specificity to eukaryotic initiation 
            factor 2alpha holophosphatases.
  JOURNAL   Elife. 2015 Mar 16;4. doi: 10.7554/eLife.04871.
  PUBMED    25774600
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-03-16"; volume: "4. doi"; pages: " 10.7554/eLife.04871"
COMMENT     Sequence Label: FLAG_hPPP1R15B_4-713_mCherry_UK1298
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctcg tttagtgaac cgtcagaatt gatctaccat ggactacaaa gacgatgacg
      121 acaagcttgg gacaggcgga tcgcggaaac ggcttggccc tcgggcgggc ttccggttct
      181 ggccaccctt tttccctcgg cgatcgcaag caggctcttc taagttcccg acgcctcttg
      241 gcccggaaaa ctccgggaac cccacactgc tttcctctgc ccagcccgag actcgggtca
      301 gttactggac gaaactgctc tcccagctcc ttgcgccgct ccccggattg cttcagaagg
      361 tgctaatttg gagccaactt ttcggtggaa tgtttccgac cagatggcta gattttgctg
      421 gagtctacag cgccctgaga gccctgaagg gacgggagaa accagccgcc cccacagcgc
      481 agaaatcttt gagttcgctg cagctcgact cctcagaccc ctcggtcacc agtccccttg
      541 attggctaga ggaggggatc cactggcaat actcgccccc agacctaaaa ttggagctta
      601 aggccaaggg aagtgctttg gaccctgcag cacaggcttt tctcttagag cagcagctgt
      661 ggggagtgga gctgttgccc agtagccttc aatcccgtct gtactctaac cgggaacttg
      721 gctcttcgcc ctctgggcct ctaaacattc aacgcataga caatttcagt gtggtatcct
      781 atttgctgaa cccttcctac ctggactgct ttcctaggct agaagtcagc tatcagaaca
      841 gtgatggaaa tagcgaggta gtcggcttcc agacactaac cccagagagc agctgcctga
      901 gagaggacca ttgtcatccc cagccgctga gtgcagaact cattccggcc tcgtggcagg
      961 gatgtccac
//
