LOCUS       sequence-158175-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 
            4-713).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Chen R, Rato C, Yan Y, Crespillo-Casado A, Clarke HJ, Harding HP, 
            Marciniak SJ, Read RJ, Ron D
  TITLE     G-actin provides substrate-specificity to eukaryotic initiation 
            factor 2alpha holophosphatases.
  JOURNAL   Elife. 2015 Mar 16;4. doi: 10.7554/eLife.04871.
  PUBMED    25774600
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-03-16"; volume: "4. doi"; pages: " 10.7554/eLife.04871"
COMMENT     Sequence Label: FLAG_hPPP1R15B_4-713_mCherry_UK1298
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catggtggcg
       61 atggatctac cgtcgacaca ttgcttgaga acattaagtc ctttgaagca tgttccctgg
      121 agtctattaa acattctttc tctgtgttca aatgtcaagc aatatccaat agcatcttct
      181 gtttcttgaa ttcgtttctg gaacctgcat ccatcccttg caaattcttc ccatggtcct
      241 ttgcgatcct catcaccact tatataatac tcagtaactt cttcaaggaa ggttaccttt
      301 tttcttttga catgtgtgtg tcttcctcca gaaagaacgt cacgctgtac cgagtctgga
      361 cattcacttt cttggctccc caacagctgc accttacaag aaagtaaggt gtgacactca
      421 gaaatggcca caatggactc agatggggtc tttgagtcac gacagccttt ctcattttcc
      481 cctgatgttt gaaaaggagc cttaaaattt aaagggttgt aggggtcatc agaattacag
      541 aatgagttcc acagtttgag actctctgct tcatctgcac tagattccca gtcatcttcc
      601 tccccagaac tatgctcagg ggtctcagga aggcttccag actgggagga attctctaga
      661 tcagacttgc cagacaaatc cttctctgaa tcagaaggct cttcaggaac aattctggca
      721 gcagtctgaa ttgttgctgt aaagttctgg ggattataag gatctacact gcagaaagag
      781 ttccaaaggt gaagcccttc agggtcttgt tcaaggtctg agtctgacag tgagctatca
      841 ctatcaaaac catcatcctc agcttcctca tcccaatcct caccttctgg atcagaactt
      901 gtttccaggt cactggatgc acctcccaaa atataatcta tcagtttgtt actacaagct
      961 ggtctggcag aaatgggaag gtcaccttct aggtatgagt aatcaactac acttattcgg
     1021 c
//
