LOCUS       sequence-158177-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty lentiviral backbone with the MNDU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Logan AC, Nightingale SJ, Haas DL, Cho GJ, Pepper KA, Kohn DB
  TITLE     Factors influencing the titer and infectivity of lentiviral vectors.
  JOURNAL   Hum Gene Ther. 2004 Oct;15(10):976-88.
  PUBMED    15585113
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2004-10"; volume: "15(10)"; pages: "976-88"
COMMENT     Sequence Label: pCCL-c-MNDU3-X
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(256..300)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    485..718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
       61 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
      121 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
      181 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
      241 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
      301 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
      361 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
      421 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
      481 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
      541 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
      601 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
      661 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
      721 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
      781 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
      841 atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt
      901 tttgctcttc ctcta
//
