LOCUS       sequence-158181-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     Lentiviral vector for constitutive gene expression.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: N174-MCS
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(61..855)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(61..855)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(172..191)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     782..801
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaatctttc acaaattttg taatccagag gttgattgtt ccagacgcgc cccgcccggg
       61 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
      121 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
      181 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
      241 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
      301 gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc
      361 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
      421 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
      481 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
      541 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
      601 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
      661 cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
      721 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
      781 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
      841 atcttgttca atcatggtcc taggaagctt gggctgcagg tcgaaaggcc cggagatgag
      901 gaagaggaga acagcgcg
//
