LOCUS       sequence-158183-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  TITLE     Lentiviral vector for constitutive gene expression.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: N174-MCS (Puro)
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..49
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     intron          152..355
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(175..195)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc
       61 ttgggaaaag cgcctcccct acccggtaga attggatcca cgcgttgtac aatgcatgcg
      121 gccgcttcga agaattcgat cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca
      181 ctgtctgagg cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga
      241 attacatcaa gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact
      301 ccatcgcata aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact
      361 aatcgaggtg cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg
      421 acggaaaggc ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg
      481 tcctccattt tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa
      541 ctggtgccga ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc
      601 caggcaccag agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg
      661 ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg
      721 cggccccaaa aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt
      781 gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga
      841 aagcagcgag acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag
      901 gccaggcctc aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct
      961 cccacccac
//
