LOCUS       sequence-158236-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a CRISPR sgRNA that allows for mutation of the surface 
            antigen SAG1 in Toxoplasma gondii and confers resistance to 
            pyrimethamine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, 
            Saeij JP, Carruthers VB, Niles JC, Lourido S
  TITLE     A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential 
            Apicomplexan Genes.
  JOURNAL   Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 
            10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
  PUBMED    27594426
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08.019"; journalName: "Cell"; date: "2016-09-8-
            8"; volume: "166"; issue: "6"; pages: "1423-1435.e12"
COMMENT     Sequence Label: pU6-SAG1-DHFR
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        635..710
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgggatgag
      121 acaaagtgcg cgagttgaaa tcgtcgtggg gacgatttca ccgcggccac atgttggaga
      181 cactgagggc acacgggaaa cgcgaaagat ttcaaattaa cgtacccaaa cgcgaaagct
      241 tgcgcagcat acactcgaag cgaacatccc gaaccatcga gaggcggaga gcgataagtc
      301 tttcacgctg cgaagtgttg cgacggctgc gccgctgcac tgtgaattgg gcgccaatat
      361 tgcatcctag gcctgacgcg cctcctgcag aacgcgagac actgggatat gtagagccaa
      421 gggggaaacc ttcgaactct cgaatgtctt ctctgacaag aatcatattt ccatcagttc
      481 tgtcagattt tcaaatggcg acctgcagag gcctgcttcc tccctgtgcg ctcttcgaag
      541 gggctttctg tcgcgcaggg tcacctcgtc cccgaagggg gtgtttgcct tctggtaaat
      601 ggggatgtca agttggcagt gagacgcgcc gtcagtttta gagctagaaa tagcaagtta
      661 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttttc
      721 tttttctcta gaggtaccat gcatctagca tgtcattcga ttttcacccc ccgcgtagtt
      781 cctgtgtgtc attcgttgtc gagacaactc tgtcccgccc cggtgctgtt ccatatgcgt
      841 gacttt
//
