LOCUS       sequence-158240-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint target selector TUBB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label:  pCAS9-mCherry-TUBB
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             273..326
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
       61 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      121 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
      181 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
      241 tgtacaaggt cgctaccatc gagggatccg gagagggcag aggaagtctg ctaacatgcg
      301 gtgacgtcga ggagaatcct ggcccaatgg acaagaagta cagcatcggc ctggacatcg
      361 gcaccaacag cgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaagt
      421 tcaaggtgct gggcaacacc gaccgccaca gcatcaagaa gaacctgatc ggcgccctgc
      481 tgttcgacag cggcgagacc gccgaggcca cccgcctgaa gcgcaccgcc cgccgccgct
      541 acacccgccg caagaaccgc atctgctacc tgcaggagat cttcagcaac gagatggcca
      601 aggtggacga cagcttcttc caccgcctgg aggagagctt cctggtggag gaggacaaga
      661 agcacgagcg ccaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
      721 accccaccat ctaccacctg cgcaagaagc tggtggacag caccgacaag gccgacctgc
      781 gcctgatcta cctggccctg gcccacatga tcaagt
//
