LOCUS       sequence-158241-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint target selector TUBB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label:  pCAS9-mCherry-TUBB
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     159..181
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     197..213
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        240..488
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     240..260
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     411..430
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        509..584
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
       61 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
      121 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
      181 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccg
      241 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      301 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      361 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      421 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      481 gaaacaccgg gtgaggaggc cgaagagggt tttagagcta gaaatagcaa gttaaaataa
      541 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tcgaattccg
      601 atgtacgggc cagatatacg cgttgacatt gattattgtc tagttattaa tagtaatcaa
      661 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      721 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      781 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      841 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc c
//
