LOCUS       sequence-158248-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint target selector TUBB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label:  pCAS9-mCherry-TUBB
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..27)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcgctaacc ttgcgcttct tcttggggtc ggcgcggctg tcgccgccca gctggctcag
       61 gtcgatgcgg gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac
      121 ctccttggtg ctggtgtagc gcttgcggtc gatggtggtg tcgaagtact tgaaggcggc
      181 gggggcgccc aggttggtca gggtgaacag gtggatgatg ttctcggcct gctcgcggat
      241 gggcttgtcg cggtgcttgt tgtaggcgct cagcaccttg tccaggttgg cgtcggccag
      301 gatcacgcgc ttgctgaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg
      361 ctgctccacg aacagctgct tctgctcgtt gtcctcgggg ctgcccttca gcttctcgta
      421 gtggctggcc aggtacagga agttcacgta cttgctgggc agggccagct cgttgccctt
      481 ctgcagctcg ccggcgctgg ccagcatgcg cttgcggccg ttctccagct cgaacaggct
      541 gtacttgggc agcttgatga tcaggtcctt cttcacctcc ttgtagccct tggcctccag
      601 gaagtcgatg gggttcttct cgaagctgct gcgctccatg atggtgatgc ccagcagctc
      661 cttcacgctc ttcagcttct tgctcttgcc cttctccacc ttggccacca ccagcacgct
      721 gtaggccacg gtggggctgt cgaagccgcc gtacttcttg gggtcccagt ccttcttgcg
      781 ggcgatcagc ttgtcgctgt tgcgcttggg
//
