LOCUS       sequence-158257-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-2A-TurboGFP-PEST-PuroR
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..96
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             103..798
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggagcg gcgagggcag aggaagtctg
       61 ctaacatgcg gtgacgtcga ggagaatcct ggcccaggat ccatggagag cgacgagagc
      121 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
      181 gagctggtgg gcggcggaga gggcaccccc gagcagggcc gcatgaccaa caagatgaag
      241 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
      301 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
      361 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
      421 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt gatgggcacc
      481 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
      541 gagcacctgc accccatggg cgataacgat ctggatggca gcttcacccg caccttcagc
      601 ctgcgcgacg gcggctacta cagctccgtg gtggacagcc acatgcactt caagagcgcc
      661 atccacccca gcatcctgca gaacggcggc cccatgttcg ccttccgccg cgtggaggag
      721 gatcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gaccccggat
      781 gcagatgccg gtgaagaaag atctcgagat atcagccatg gcttcccgcc ggcggtggcg
      841 gcgc
//
