LOCUS       sequence-158258-        4956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, 
            H840A ) protein with a human AID mutant protein (p182X). MSCV 
            Retroviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X
  TITLE     Targeted AID-mediated mutagenesis (TAM) enables efficient genomic 
            diversification in mammalian cells.
  JOURNAL   Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. 
            Epub 2016 Oct 10.
  PUBMED    27723754
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4027"; journalName: "Nat Methods"; date: "2016-12"; 
            volume: "13"; issue: "12"; pages: "1029-1035"
COMMENT     Sequence Label: MO91-dCas9-AID(P182X)
FEATURES             Location/Qualifiers
     source          1..4956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..78
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             85..105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4246..4266
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4282..4302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4924..4947
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agatctacca tggactataa ggaccacgac ggagactaca aggatcatga tattgattac
       61 aaagacgatg acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc
      121 ccagcagcta ccatggacaa gaagtattct atcggactgg ccatcgggac taatagcgtc
      181 gggtgggccg tgatcactga cgagtacaag gtgccctcta agaagttcaa ggtgctcggg
      241 aacaccgacc ggcattccat caagaaaaat ctgatcggag ctctcctctt tgattcaggg
      301 gagaccgctg aagcaacccg cctcaagcgg actgctagac ggcggtacac caggaggaag
      361 aaccggattt gttaccttca agagatattc tccaacgaaa tggcaaaggt cgacgacagc
      421 ttcttccata ggctggaaga atcattcctc gtggaagagg ataagaagca tgaacggcat
      481 cccatcttcg gtaatatcgt cgacgaggtg gcctatcacg agaaataccc aaccatctac
      541 catcttcgca aaaagctggt ggactcaacc gacaaggcag acctccggct tatctacctg
      601 gccctggccc acatgatcaa gttcagaggc cacttcctga tcgagggcga cctcaatcct
      661 gacaatagcg atgtggataa actgttcatc cagctggtgc agacttacaa ccagctcttt
      721 gaagagaacc ccatcaatgc aagcggagtc gatgccaagg ccattctgtc agcccggctg
      781 tcaaagagcc gcagacttga gaatcttatc gctcagctgc cgggtgaaaa gaaaaatgga
      841 ctgttcggga acctgattgc tctttcactt gggctgactc ccaatttcaa gtctaatttc
      901 gacctggcag aggatgccaa gctgcaactg tccaaggaca cctatgatga cgatctcgac
      961 aacctcctgg cccagatcgg tgaccaatac gccgaccttt tccttgctgc taagaatctt
     1021 tctgacgcca tcctgctgtc tgacattctc cgcgtgaaca ctgaaatcac caaggcccct
     1081 ctttcagctt caatgattaa gcggtatgat gagcaccacc aggacctgac cctgcttaag
     1141 gcactcgtcc ggcagcagct tccggagaag tacaaggaaa tcttctttga ccagtcaaag
     1201 aatggatacg ccggctacat cgacggaggt gcctcccaag aggaatttta taagtttatc
     1261 aaacctatcc ttgagaagat ggacggcacc gaagagctcc tcgtgaaact gaatcgggag
     1321 gatctgctgc ggaagcagcg cactttcgac aatgggagca ttccccacca gatccatctt
     1381 ggggagcttc acgccatcct tcggcgccaa gaggacttct acccctttct taaggacaac
     1441 agggagaaga ttgagaaaat tctcactttc cgcatcccct actacgtggg acccctcgcc
     1501 agaggaaata gccggtttgc ttggatgacc agaaagtcag aagaaactat cactccctgg
     1561 aacttcgaag aggtggtgga caagggagcc agcgctcagt cattcatcga acggatgact
     1621 aacttcgata agaacctccc caatgagaag gtcctgccga aacattccct gctctacgag
     1681 tactttaccg tgtacaacga gctgaccaag gtgaaatatg tcaccgaagg gatgaggaag
     1741 cccgcattcc tgtcaggcga acaaaagaag gcaattgtgg accttctgtt caagaccaat
     1801 agaaaggtga ccgtgaagca gctgaaggag gactatttca agaaaattga atgcttcgac
     1861 tctgtggaga ttagcggggt cgaagatcgg ttcaacgcaa gcctgggtac ctaccatgat
     1921 ctgcttaaga tcatcaagga caaggatttt ctggacaatg aggagaaaga ggacatcctt
     1981 gaggacattg tcctgactct cactctgttc gaggaccggg aaatgatcga ggagaggctt
     2041 aagacctacg cccatctgtt cgacgataaa gtgatgaagc aacttaaacg gagaagatat
     2101 accggatggg gacgccttag ccgcaaactc atcaacggaa tccgggacaa acagagcgga
     2161 aagaccattc ttgatttcct taagagcgac ggattcgcta atcgcaactt catgcaactt
     2221 atccatgatg attccctgac ctttaaggag gacatccaga aggcccaagt gtctggacaa
     2281 ggtgactcac tgcacgagca tatcgcaaat ctggctggtt cacccgctat taagaagggt
     2341 attctccaga ccgtgaaagt cgtggacgag ctggtcaagg tgatgggtcg ccataaacca
     2401 gagaacattg tcatcgagat ggccagggaa aaccagacta cccagaaggg acagaagaac
     2461 agcagggagc ggatgaaaag aattgaggaa gggattaagg agctcgggtc acagatcctt
     2521 aaagagcacc cggtggaaaa cacccagctt cagaatgaga agctctatct gtactacctt
     2581 caaaatggac gcgatatgta tgtggaccaa gagcttgata tcaacaggct ctcagactac
     2641 gacgtggacg ccatcgtccc tcagagcttc ctcaaagacg actcaattga caataaggtg
     2701 ctgactcgct cagacaagaa ccggggaaag tcagataacg tgccctcaga ggaagtcgtg
     2761 aaaaagatga agaactattg gcgccagctt ctgaacgcaa agctgatcac tcagcggaag
     2821 ttcgacaatc tcactaaggc tgagaggggc ggactgagcg aactggacaa agcaggattc
     2881 attaaacggc aacttgtgga gactcggcag attactaaac atgtagccca aatccttgac
     2941 tcacgcatga ataccaagta cgacgaaaac gacaaactta tccgcgaggt gaaggtgatt
     3001 accctgaagt ccaagctggt cagcgatttc agaaaggact ttcaattcta caaagtgcgg
     3061 gagatcaata actatcatca tgctcatgac gcatatctga atgccgtggt gggaaccgcc
     3121 ctgatcaaga agtacccaaa gctggaaagc gagttcgtgt acggagacta caaggtctac
     3181 gacgtgcgca agatgattgc caaatctgag caggagatcg gaaaggccac cgcaaagtac
     3241 ttcttctaca gcaacatcat gaatttcttc aagaccgaaa tcacccttgc aaacggtgag
     3301 atccggaaga ggccgctcat cgagactaat ggggagactg gcgaaatcgt gtgggacaag
     3361 ggcagagatt tcgctaccgt gcgcaaagtg ctttctatgc ctcaagtgaa catcgtgaag
     3421 aaaaccgagg tgcaaaccgg aggcttttct aaggaatcaa tcctccccaa gcgcaactcc
     3481 gacaagctca ttgcaaggaa gaaggattgg gaccctaaga agtacggcgg attcgattca
     3541 ccaactgtgg cttattctgt cctggtcgtg gctaaggtgg aaaaaggaaa gtctaagaag
     3601 ctcaagagcg tgaaggaact gctgggtatc accattatgg agcgcagctc cttcgagaag
     3661 aacccaattg actttctcga agccaaaggt tacaaggaag tcaagaagga ccttatcatc
     3721 aagctcccaa agtatagcct gttcgaactg gagaatgggc ggaagcggat gctcgcctcc
     3781 gctggcgaac ttcagaaggg taatgagctg gctctcccct ccaagtacgt gaatttcctc
     3841 taccttgcaa gccattacga gaagctgaag gggagccccg aggacaacga gcaaaagcaa
     3901 ctgtttgtgg agcagcataa gcattatctg gacgagatca ttgagcagat ttccgagttt
     3961 tctaaacgcg tcattctcgc tgatgccaac ctcgataaag tccttagcgc atacaataag
     4021 cacagagaca aaccaattcg ggagcaggct gagaatatca tccacctgtt caccctcacc
     4081 aatcttggtg cccctgccgc attcaagtac ttcgacacca ccatcgaccg gaaacgctat
     4141 acctccacca aagaagtgct ggacgccacc ctcatccacc agagcatcac cggactttac
     4201 gaaactcgga ttgacctctc acagctcgga ggggatgagg gagctcccaa gaaaaagcgc
     4261 aaggtaggta gttccggatc tccgaaaaag aaacgcaaag ttggtagtga tgctttagac
     4321 gattttgact tagatatgct tggttcagac gcgttagacg acttcggtgg aggatccatg
     4381 gacagcctct tgatgaaccg gaggaagttt ctttaccaat tcaaaaatgt ccgctgggct
     4441 aagggtcggc gtgagaccta cctgtgctac gtagtgaaga ggcgtgacag tgctacatcc
     4501 ttttcactgg actttggtta tcttcgcaat aagaacggct gccacgtgga attgctcttc
     4561 ctccgctaca tctcggactg ggacctagac cctggccgct gctaccgcgt cacctggttc
     4621 acctcctgga gcccctgcta cgactgtgcc cgacatgtgg ccgactttct gcgagggaac
     4681 cccaacctca gtctgaggat cttcaccgcg cgcctctact tctgtgagga ccgcaaggct
     4741 gagcccgagg ggctgcggcg gctgcaccgc gccggggtgc aaatagccat catgaccttc
     4801 aaagattatt tttactgctg gaatactttt gtagaaaacc atgaaagaac tttcaaagcc
     4861 tgggaagggc tgcatgaaaa ttcagttcgt ctctccagac agcttcggcg catccttttg
     4921 cccgattaca aagacgatga cgataagtga ctcgag
//
