LOCUS       sequence-158269-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-AviTag-PuroR
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..104
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             114..167
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(802..821)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     856..875
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gatagctagc gggccagtac ccaaaaagcg gggggtctgg tggcagtgga gggggatccg
       61 gcctgaacga catcttcgag gcccagaaga tcgagtggca cgagtccgga ggagagggca
      121 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg actgaataca
      181 agcctactgt caggttggct acaagagacg acgttcctag agccgtgaga actctggctg
      241 cagccttcgc cgactacccc gccacgagac acaccgttga cccagatcgg catattgaga
      301 gagtgactga actgcaggag ctgtttctta caagagttgg cctcgacata ggcaaggtgt
      361 gggtggcgga cgacggcgcc gccgtggccg tctggaccac tcccgaatca gttgaggctg
      421 gcgccgtatt cgctgagatc ggcccgagaa tggctgagct cagcgggagt aggctcgcgg
      481 cacagcagca aatggaggga ctgctggcac cacacaggcc caaagaaccc gcctggttcc
      541 tggcaaccgt cggtgtatct cccgatcatc aggggaaagg tctgggctct gccgtagtgc
      601 tccctggcgt ggaggcagct gagagagcag gagtacctgc cttcttggag acctccgctc
      661 caaggaatct tcccttctat gaacggttgg gcttcaccgt gacagccgac gtggaagtcc
      721 ccgaaggccc ccgcacttgg tgcatgacga ggaagcctgg agcgtgaggg cccgcttcga
      781 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      841 aaatgcttta tttgtgaaat ttgtgatgct attgcttt
//
