LOCUS       sequence-158270-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  TITLE     Shivdasani lab plasmids
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p140 pETF-GST RanBP10 L301G
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..51
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(114..137)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(147..176)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag
       61 ccaactcagc ttcctttcgg gctttgttta gcagcctagg tattaatcaa ttagtggtgg
      121 tggtggtggt ggtggtgctc gagctacaga tcctcttctg agatgagttt ttgttctgcg
      181 gggtatttac tgtggttctg tttgttcttg gtggatgcga cgccattgct gcagctggga
      241 ctgtctgctc ctgtgttgtg tatatgggaa ctactggggc catgtcgggg gctgaggctg
      301 ggggagccag ggtagctgtc ctgggacttg gggcttcggg aactcaggct gcggacctca
      361 ctgtcagtgc cgttcaccat ctccacgaac tgtcgacact tgagcatgaa gagcaggttg
      421 ggattatgtt ccagcagccc agggtagaag cgctgggttg tctcaatggc ttcacccacc
      481 cggccttcca gcaccccctt ctggattttt tgtctgttct ttatggatgc ctgctcttcc
      541 tgaatcgggg tttcagtcat tcgggcaaaa gctgtggctg tggaacaata cccatgatgc
      601 accaggtaag acgagaccat gttctgcagc accgcctgcc actcgccaag ccgggcactt
      661 atgggaaagc cgtgcacagt gccctggact ttggcacgcc actcccgcat atagtcctca
      721 atgtcgaaca ggaagggctg ctgcccaaag ttggcatcca caatctcccc
//
