LOCUS       sequence-158271-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-AviTag-PuroR
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(604..657)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(667..711)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     misc_feature    775..866
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(807..826)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    880..928
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ccctcacgct ccaggcttcc tcgtcatgca ccaagtgcgg gggccttcgg ggacttccac
       61 gtcggctgtc acggtgaagc ccaaccgttc atagaaggga agattccttg gagcggaggt
      121 ctccaagaag gcaggtactc ctgctctctc agctgcctcc acgccaggga gcactacggc
      181 agagcccaga cctttcccct gatgatcggg agatacaccg acggttgcca ggaaccaggc
      241 gggttctttg ggcctgtgtg gtgccagcag tccctccatt tgctgctgtg ccgcgagcct
      301 actcccgctg agctcagcca ttctcgggcc gatctcagcg aatacggcgc cagcctcaac
      361 tgattcggga gtggtccaga cggccacggc ggcgccgtcg tccgccaccc acaccttgcc
      421 tatgtcgagg ccaactcttg taagaaacag ctcctgcagt tcagtcactc tctcaatatg
      481 ccgatctggg tcaacggtgt gtctcgtggc ggggtagtcg gcgaaggctg cagccagagt
      541 tctcacggct ctaggaacgt cgtctcttgt agccaacctg acagtaggct tgtattcagt
      601 cattgggcca ggattctcct cgacgtcacc gcatgttagc agacttcctc tgccctctcc
      661 tccggactcg tgccactcga tcttctgggc ctcgaagatg tcgttcaggc cggatccccc
      721 tccactgcca ccagaccccc cgctttttgg gtactggccc gctagctatc attaagagaa
      781 atgttctggc acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg
      841 ttttgttttg atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca
      901 gatgtaatga aaataaagat attttattgc ggcctgtcca atacctcccg taccttaata
      961 ttacttactt atccttga
//
