LOCUS       sequence-158274-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-cTEV-PuroR
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             442..495
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 aagagcatga gcagcatggt gagcgacacc agctgcacct tccccagcag cgacggcatc
      121 ttctggaagc actggattca gaccaaggac ggccagtgcg gcagccccct ggtgagcacc
      181 agagacggct tcatcgtggg catccacagc gccagcaact tcaccaacac caacaactac
      241 ttcaccagcg tgcccaagaa cttcatggag ctgctgacca accaggaggc ccagcagtgg
      301 gtgagcggct ggagactgaa cgccgacagc gtgctgtggg gcggccacaa ggtgttcatg
      361 agcaagcccg aggagccctt ccagcccgtg aaggaggcca cccagctgat gaacgagctg
      421 gtgtacagcc agtccggagg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
      481 gagaatcctg gcccaatgac tgaatacaag cctactgtca ggttggctac aagagacgac
      541 gttcctagag ccgtgagaac tctggctgca gccttcgccg actaccccgc cacgagacac
      601 accgttgacc cagatcggca tattgagaga gtgactgaac tgcaggagct gtttcttaca
      661 agagttggcc tcgacatagg caaggtgtgg gtggcggacg acggcgccgc cgtggccgtc
      721 tggaccactc ccgaatcagt tgaggctggc gccgtattcg ctgagatcgg cccgagaatg
      781 gct
//
