LOCUS       sequence-158308-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For assaying self-targeting activity via Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 1 - U6p_20nt1_WT_sgRNA
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..135
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        214..289
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(301..325)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(385..408)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
       61 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      121 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      181 aggacggaac accgtaagtc ggagtactgt cctgttttag agctagaaat agcaagttaa
      241 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctag
      301 acccagcttt cttgtacaaa gttggcatta gacgtcgagg aattctaccg ggtaggggag
      361 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      421 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      481 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
      541 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
      601 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
      661 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
      721 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
//
