LOCUS       sequence-158315-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG 
            (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
            LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
  TITLE     Real-time quantification of single RNA translation dynamics in 
            living cells.
  JOURNAL   Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
            Epub 2016 May 5.
  PUBMED    27313040
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf0899"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1425-9"
COMMENT     Sequence Label: pUB_smFLAG_ActB_MS2
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgatccaca tctgctggaa ggtggacagc gaggccagga tggagccgcc gatccacacg
       61 gagtacttgc gctcaggagg agcaatgatc ttgatcttca ttgtgctggg tgccagggca
      121 gtgatctcct tctgcatcct gtcggcaatg ccagggtaca tggtggtgcc gccagacagc
      181 actgtgttgg cgtacaggtc tttgcggatg tccacgtcac acttcatgat ggagttgaag
      241 gtagtttcgt ggatgccaca ggactccatg cccaggaagg aaggctggaa gagtgcctca
      301 gggcagcgga accgctcatt gccaatggtg atgacctggc cgtcaggcag ctcgtagctc
      361 ttctccaggg aggagctgga agcagccgtg gccatctctt gctcgaagtc cagggcgacg
      421 tagcacagct tctccttaat gtcacgcacg atttcccgct cggccgtggt ggtgaagctg
      481 tagccgcgct cggtgaggat cttcatgagg tagtcagtca ggtcccggcc agccaggtcc
      541 agacgcagga tggcatgggg gagggcatac ccctcgtaga tgggcacagt gtgggtgacc
      601 ccgtcaccgg agtccatcac gatgccagtg gtacggccag aggcgtacag ggatagcaca
      661 gcctggatag caacgtacat ggctggggtg ttgaaggtct caaacatgat ctgggtcatc
      721 ttctcgcggt tggccttggg gttcaggggg gcctcggtca gcagcacggg gtgctcctcg
      781 ggagccacac gcagctcatt gtagaaggtg tggtgccaga ttttctccat gtcgtcccag
      841 ttggtgacga tgccgtgctc gatggggtac ttcagggtga ggatgcctct cttgctctgg
      901 gcctcgtcgc ccacatagga atccttctga cccatgccca ccatcacgcc ctggtgcctg
      961 gggcgcccca cgatggaggg gaagacggcc cggggggcat cgtcgcccgc gaagccggcc
     1021 ttgcacatgc cggagccgt
//
