LOCUS       sequence-158316-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG 
            (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
            LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
  TITLE     Real-time quantification of single RNA translation dynamics in 
            living cells.
  JOURNAL   Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
            Epub 2016 May 5.
  PUBMED    27313040
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf0899"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1425-9"
COMMENT     Sequence Label: pUB_smFLAG_ActB_MS2
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      70..79
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             79..102
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             133..156
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             661..684
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             691..714
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             727..750
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             757..780
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
       61 ttggcggccg ccaccatgga ctacaaggac gacgacgaca aaggtgacta caaagatgat
      121 gacgataaag gcgactataa ggacgatgac gacaagggcg gaaactcact gatcaaggaa
      181 aacatgcgga tgaaggtggt gatggagggc tccgtgaatg gtcaccagtt caagtgcacc
      241 ggagagggag agggaaaccc gtacatggga actcagacca tgcgcattaa ggtcatcgaa
      301 ggaggtccgc tgccgttcgc tttcgatatc ctggccactt cgttcggagg agggtcgcgc
      361 acgttcatca agtacccgaa gggaatcccg gacttcttta agcagtcatt cccggaagga
      421 ttcacttggg aacgggtgac ccggtatgaa gatggaggtg tggtgactgt catgcaagat
      481 acttcgctgg aggatgggtg cctcgtgtac cacgtccaag tccgcggagt gaatttcccg
      541 tccaacggac cagtgatgca gaaaaagacg aagggttggg aacctaatac tgaaatgatg
      601 taccccgcag acggagggct gaggggctac acccacatgg cgctgaaggt cgacggagga
      661 gattacaagg atgacgacga taagcaacaa gattacaaag acgatgatga caagggccag
      721 cagggcgact acaaggacga cgacgacaag cagcaggact acaaagatga cgatgataaa
      781 ggaggaggac atctgtcctg ttcgttcgtg accacctaca gatcaaagaa aaccgtggga
      841 aacatcaaga tgccgggcat tcatgccgtc gaccaccgcc tggagcggct cgaagaatca
      901 gacaatgaga tgttcgtcgt gcaaagagaa catgccgtgg ccaagttcgc gggactggga
      961 ggcggtggag gcgattacaa agacgatgat gac
//
