LOCUS       sequence-158317-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG 
            (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
            LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
  TITLE     Real-time quantification of single RNA translation dynamics in 
            living cells.
  JOURNAL   Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
            Epub 2016 May 5.
  PUBMED    27313040
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf0899"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1425-9"
COMMENT     Sequence Label: pUB_smFLAG_ActB_MS2
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caaattttgt aatccagagg ttgattagga tctatcgata tgagatctga ggtgtttgat
       61 gtacggtgcg gtgatcctgc accgtttgcc tggttgtaag tttgtgggtt gtcggcggat
      121 gccgtgttcg tgcatccgtt tgtcgtgttt ttgtgaggtt ttccgccgcg ggaaggctgt
      181 tgctcttccc gtcggatgtt tttcgtaata gtttgcgctg gacgaaagcg tgatcgtctt
      241 tcgtatttgt aaaccagttg atttttccgt gtgtaccgtt cgctgttgct gaacggtttg
      301 gtttttgata gtagtgagag atgtgggctg gtacgctgat gctgtaccag taattgtttt
      361 tgatttggta tgttggaatg ggcaagcggt gatcctgctt gccagttttg tgggaagagg
      421 tttgaggtag gagtgggttc gctggtgctg aacccatttg gtagtttctg tggttgagac
      481 ttgtggagca tgccgtgatc gtgcatgctt gaatgtcctg agtggaagtg atgtagctgc
      541 gggatccgtg atcgtgatcc cgaaaggtaa gtaatcgtat tggacgtgga acgacgagcc
      601 ctggtggtgc tcgtccttgt ccgtgttttg aaattcgtga gagcatgggt ggcgtgatcg
      661 tccacccaag aaataggcac aatgtatgct cgagtgtttc gaagcgctga tgctgcttcg
      721 agaagagtag tttttgtctt gttggtgaga gtccatggcc tgatggtcca tggagggttt
      781 gtccagttgg acaagaaaca acactccgag ccctgttggt gctcggatgt gattttgttg
      841 gggatgtatt cttggggccc atgcgctgat gctgcatggg gacataaatt ttcttgagtt
      901 gggtactggg caggaacgct gatgctgttc ctgta
//
